长春市沙门菌耐药特性及相关分子特征的分析

Analysis of drug resistance and related molecular characteristics of Salmonella in Changchun City

目的为掌握长春市沙门菌耐药特性及监测长春市沙门菌耐药基因的传播方式,并提供一定的理论基础。方法根据微量肉汤稀释法测定沙门菌的最低抑菌浓度(minimal inhibitory concentration,MIC)。利用全基因组测序技术及生物信息学方法分析沙门菌耐药相关分子特征。结果105株沙门菌对链霉素(STR)、氨苄西林(AMP)、四环素(TET)、萘啶酸(NAL)、氨苄西林/舒巴坦(AMS)、氯霉素(CHL)耐药性较高,对阿米卡星(AMI)、头孢他啶/阿维巴坦(CZA)、厄他培南(ETP)、多黏菌素E、环丙沙星(CIP)敏感性较高。长春市沙门菌多重耐药现象严重,耐药模式多样。针对不同的抗生素耐药表型与耐药基因差异较大。质粒、转座子与耐药基因之间关系密切。结论长春市沙门菌耐药情况严重,以获得性耐药为主。

Objective This paper aims to provide a theoretical basis for understanding the drug resistance characteristics of Salmonella in Changchun City and monitoring the transmission mode of drug resistance genes.Methods Minimal inhibitory concentration(MIC)of Salmonella was determined by microdilution of broth method.Related molecular characterization of drug resistance in Salmonella was analyzed by whole genome sequencing and bioinformatics methods.Results A total of 105 strains of Salmonella were highly resistant to streptomycin(STR),ampicillin(AMP),tetracycline(TET),nalidixate(NAL),ampicillin/sulbactam(AMS),chloramphenicol(CHL),and highly sensitive to amikacin(AMI),ceftazidine/avibactam(CZA),ertabenem(ETP),polymyxin E,ciprofloxacin(CIP).The multi-drug resistance of Salmonella in Changchun was serious,and there were various drug resistance patterns.Different antibiotic resistance phenotypes and resistance genes differ greatly.Plasmids,transposons and drug resistance genes are closely related.Conclusion The resistance of Salmonella in Changchun was serious,and acquired drug resistance was predominant.