轮状病毒肠炎患儿肝功能与TLR4/NF-κB p65通路的关系

Relationship between liver function and TLR4/NF-κB p65 pathway of children with rotavirus enteritis

目的探究轮状病毒(RV)肠炎患儿肝功能损伤情况及其与Toll样受体4/核转录因子κB p65(TLR4/NF-κB p65)通路的关系。方法选取2019年1月-2021年6月医院收治的110例RV感染阳性的肠炎患儿为RV感染组,另选取同期收治的80例RV感染阴性的肠炎患儿为对照组,采用全自动生化分析仪检测患儿肝功能[谷草转氨酶(AST)、谷丙转氨酶(ALT)],行聚合酶链式反应检测TLR4 mRNA、NF-κB mRNA相对表达量,采用蛋白质印迹法检测外周血白细胞NF-κB p65蛋白表达水平,采用酶联免疫吸附法检测血清肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平,分析肝功能与TLR4/NF-κB p65通路的关系。结果RV感染组患儿ALT、AST水平和肝损伤率均高于对照组(P<0.05);RV感染组患儿TLR4 mRNA、NF-κB mRNA、NF-κB p65蛋白表达量和血清TNF-α、IL-1β、IL-6水平均高于对照组(P<0.05);RV感染肠炎患儿ALT、AST水平均与TLR4 mRNA、NF-κB mRNA、NF-κB p65蛋白表达量和血清TNF-α、IL-1β、IL-6水平正相关(ALT:r=0.483,0.564,0.416,0.348,0.560,0.314,P<0.05;AST:r=0.537,0.457,0.312,0.434,0.572,0.399,P<0.05)。结论RV感染肠炎患儿易出现肝损害,并存在TLR4/NF-κB p65通路过度激活,且肝损害与TLR4/NF-κB p65通路过度激活有关。

OBJECTIVE To explore the liver function damage in enteritis children with rotavirus(RV)and and its relationship with Toll-like receptor 4/nuclear factor kappa B p65(TLR4/NF-κB p65)pathway.METHODS Total of 110 children with enteritis infected with RV admitted to the hospital between Jan 2019 and Jun 2021 were enrollled as the RV infection group,and another 80 children with enteritis and negative RV infection treated during the same period were enrolled as the control group.Automatic biochemical analyzer was used to detect the liver function of the children,such as aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels.And polymerase chain reaction was performed to detect the relative expression levels of TLR4 mRNA and NF-κB mRNA,and Western blot was adopted to detect the protein expression level of NF-κB p65 of leukocytes in peripheral blood.Enzyme-linked immunosorbent assay was applied to detect the levels of serum tumor necrosis factor(TNF-α),interleukin-1β(IL-1β)and IL-6,and the relationship between liver function and TLR4/NF-κB p65 pathway was analyzed.RESULTS The levels of ALT and AST and liver injury rate in the RV infection group were higher than those in the control group(P<0.05).The expression levels of TLR4 mRNA,NF-κB mRNA and NF-κB p65 protein and levels of serum TNF-α,IL-1βand IL-6 were higher in the RV infection group than those in the control group(P<0.05).The levels of ALT and AST in children with enteritis and RV infection were positively correlated with the expression levels of TLR4 mRNA,NF-κB mRNA and NF-κB p65 protein and levels of serum TNF-α,IL-1βand IL-6(ALT:r=0.483,0.564,0.416,0.348,0.560,0.314,P<0.05;AST:0.537,0.457,0.312,0.434,0.572,0.399,P<0.05).CONCLUSION Children with enteritis infected with RV are prone to have liver injury,which is related to the excessive activation of TLR4/NF-κB p65 pathway.