熵权法结合Box-Behnken响应面法优化肿节风药材的提取工艺

Optimization of extraction process of Sarcandra glabra by combination of entropy weight method with Box-Behnken response surface method

目的优化肿节风药材的提取工艺。方法以高效液相色谱(HPLC)法测定的肿节风中迷迭香酸与异嗪皮啶含量为考察指标,对超声时间、超声温度、液料比(mL/g)、甲醇体积分数进行单因素实验。基于单因素实验结果,采用Box-Behnken响应面法设计实验,运用熵权法赋予各指标权重并计算综合评分,再以综合评分为评价指标,优化肿节风药材的提取工艺,并对优化后的提取工艺进行验证。结果肿节风药材最佳提取工艺为超声时间40 min,超声温度45℃,液料比50∶1,甲醇体积分数70%。3次验证实验结果显示,平均综合评分为0.9886,RSD为0.50%,各综合评分实际值与预测值(0.9851)之间的偏差均在±1%以内。结论本研究优化后的工艺稳定可行、重复性好,可为肿节风药材的提取提供参考。

OBJECTIVE To optimize the extraction process of Sarcandra glabra.METHODS The contents of rosmarinic acid and isofraxidin in S.glabra were determined by HPLC;ultrasonic time,ultrasonic temperature,solid-liquid ratio(mL/g)and methanol volume fraction were investigated by single factor test.Based on the results of single factor test,experimental scheme was designed by Box-Behnken response surface method,and the entropy weight method was used to assign the weight of each index and calculate the comprehensive score.Taking the comprehensive score as the evaluation index,the extraction process of S.glabra was optimized,and then optimized extraction process was verified.RESULTS The optimal extraction technology of S.glabra included ultrasonic time of 40 min,ultrasonic temperature of 45℃,liquid-solid ratio of 50∶1,methanol volume fraction of 70%.The results of 3 times of verification experiment showed that average comprehensive score was 0.9886,and the RSD was 0.50%.The deviation between the actual value and the predicted value(0.9851)of each comprehensive score was within±1%.CONCLUSIONS The optimized extraction method is stable,feasible and repeatable,which can provide reference for extraction of S.glabra.