LncRNA DARS-AS1靶向miR-758-3p促进骨肉瘤细胞增殖并抑制凋亡

LncRNA DARS-AS1 promotes osteosarcoma cell proliferation and inhibits apoptosis by targeting miR-758-3p

目的:探讨LncRNA DARS-AS1对骨肉瘤细胞增殖及凋亡的影响及其可能作用机制。方法:qRT-PCR法检测骨肉瘤组织、瘤旁组织、人成骨细胞hFOB 1.19、人骨肉瘤细胞Saos2、U2OS、HOS中LncRNA DARS-AS1、miR-758-3p的表达量;对Saos2细胞进行转染,根据不同转染物分为si-NC组、si-LncRNA DARS-AS1组、miR-NC组、miR-758-3p组、si-LncRNA DARS-AS1+anti-miR-NC组、si-LncRNA DARS-AS1+anti-miR-758-3p组;MTT法、流式细胞术分别检测细胞增殖及凋亡;双荧光素酶报告实验检测LncRNA DARS-AS1与miR-758-3p的靶向调控关系。结果:与瘤旁组织比较,骨肉瘤组织中LncRNA DARS-AS1的表达量升高(P<0.05),miR-758-3p的表达量降低(P<0.05);与hFOB 1.19细胞比较,Saos2、U2OS、HOS细胞中LncRNA DARS-AS1的表达量升高(P<0.05),miR-758-3p的表达量降低(P<0.05);与si-NC组比较,si-LncRNA DARS-AS1组细胞活力降低(P<0.05),细胞凋亡率升高(P<0.05);与miR-NC组比较,miR-758-3p组细胞活力降低(P<0.05),细胞凋亡率升高(P<0.05);LncRNA DARS-AS1能够靶向结合miR-758-3p;si-LncRNA DARS-AS1+anti-miR-758-3p组与si-LncRNA DARS-AS1+anti-miR-NC组比较,细胞活力升高(P<0.05),细胞凋亡率降低(P<0.05)。结论:干扰LncRNA DARS-AS1表达可通过靶向调控miR-758-3p表达而抑制骨肉瘤细胞增殖及诱导细胞凋亡。

Objective:To explore the effect of LncRNA DARS-AS1 on the proliferation and apoptosis of osteosarcoma cells and its possible mechanism.Methods:The expression of LncRNA DARS-AS1 and miR-758-3p in osteosarcoma tissues,adjacent tissues,human osteoblasts hFOB 1.19,human osteosarcoma cells Saos2,U2OS,and HOS were detected by qRT-PCR.Transfection of Saos2 cells was divided into si-NC group,si-LncRNA DARS-AS1 group,miR-NC group,miR-758-3p group,si-LncRNA DARS-AS1+anti-miR-NC group,si-LncRNA DARS-AS1+anti-miR-758-3p group.MTT method and flow cytometry were used to detect cell proliferation and apoptosis.The dual luciferase reporter experiment was used to detect the targeted regulation relationship between LncRNA DARS-AS1 and miR-758-3p.Results:Compared with adjacent tissues,the expression of LncRNA DARS-AS1 in osteosarcoma tissue was increased(P<0.05),while the expression of miR-758-3p was decreased(P<0.05).Compared with hFOB 1.19 cells,the expression of LncRNA DARS-AS1 in Saos2,U2OS,HOS cells was increased(P<0.05),while the expression of miR-758-3p was decreased(P<0.05).Compared with the si-NC group,the cell viability in the si-LncRNA DARS-AS1 group was decreased(P<0.05),while the apoptosis rate was increased(P<0.05).Compared with the miR-NC group,the cell viability in the miR-758-3p group was decreased(P<0.05),while the apoptosis rate was increased(P<0.05).LncRNA DARS-AS1 could target miR-758-3p.Compared with the si-LncRNA DARS-AS1+anti-miR-NC group,the cell viability in the si-LncRNA DARS-AS1+anti-miR-758-3p group was increased(P<0.05),while the cell apoptosis rate was decreased( P <0.05). Conclusion: Interfering with the expression of LncRNA DARS-AS1 could inhibit the proliferation of osteosarcoma cells and induce apoptosis by targeting the regulation of miR-758-3p expression.