山竹子素在宫颈癌中的抗肿瘤活性探讨

The research of antitumor activity of garcinol in cervical cancer

目的:探讨山竹子素(garcinol)在宫颈癌中的抗肿瘤活性。方法:采用低剂量、中剂量和高剂量的山竹子素处理宫颈癌细胞。CCK-8实验检测细胞活力。EdU实验检测细胞增殖。流式细胞术检测细胞周期和凋亡。划痕实验检测细胞迁移。Transwell实验检测细胞侵袭。构建荷瘤小鼠模型,检测山竹子素对宫颈癌细胞体内成瘤能力的影响。免疫组化检测肿瘤组织Ki-67增殖指数和凋亡水平。HE染色检测山竹子素对荷瘤小鼠心、肝、肾、脾和肺的毒性作用。结果:山竹子素呈浓度依赖性的抑制宫颈癌细胞的活力和增殖,诱导细胞周期阻滞效应和细胞凋亡,降低细胞的迁移和侵袭能力(P<0.05)。山竹子素显著抑制宫颈癌细胞的异种移植瘤的形成和生长,降低Ki-67增殖指数,提高凋亡水平(P<0.05)。山竹子素对荷瘤小鼠心、肝、肾、脾和肺无明显毒性作用。结论:山竹子素在宫颈癌中具有良好的抗肿瘤活性。

Objective:To investigate the antitumor activity of garcinol in cervical cancer.Methods:Cervical cancer cells were treated with low dose,medium dose and high dose of garcinol.Cell viability was detected by CCK-8 assay.Cell proliferation was measured by EdU assay.Cell cycle progression and apoptosis were assessed by flow cytometry.Cell migration was evaluated by wound-healing assay.Cell invasion was examined by Transwell assay.The effect of garcinol on the tumorigenicity of cervical cancer cells in vivo was detected by xenograft model assay.The Ki-67 proliferative index and apoptotic level in xenograft tumors were measured by immunohistochemical analysis.The toxic effect of garcinol on the heart,liver,kidney,spleen,and lung of tumor-bearing mice was evaluated by HE staining.Results:Garcinol significantly inhibited the growth and proliferation,induced cell cycle arrest and apoptosis,and decreased the migration and invasion of cervical cancer cells in a dose-dependent manner(P<0.05).Garcinol significantly retarded the formation and growth of xenograft tumors-derived from cervical cancer cells with downregulation of Ki-67 proliferative index and upregulation of apoptosis(P<0.05).Garcinol showed no apparent toxic effect on the heart,liver,kidney,spleen,and lung of tumor-bearing mice.Conclusion:Garcinol has remarkable antitumor activity in cervical cancer.