蒙药红花水提物的毒性观察

Toxicity of Carthamus tinctorius L.Water Extract

目的:血清动态观察蒙药红花水提物(HS)对肝、心、肾的毒性作用。方法:将蒙药红花水提物分为低、中、高剂量组及正常组,分别灌胃3个月HS,每天灌胃1次,每14 d尾部采血1次,动态观察血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、三酰甘油酯(TG)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素(Urea)、肌酐(Cr)水平的变化;3个月后处死大鼠,取肝、心、肺、肾、脾、胃、肠、脑组织做苏木精-伊红染色(HE)病理观察。结果:HS低、中、高剂量组持续用药3个月后大鼠血清GPT、GOT、TG、LDH、CK、Urea、Cr水平和组织病理切片结果发现对肝、心、肾基本无损伤。结论:根据血清GPT、GOT、TG、CK、LDH、Gr、Urea水平和组织病理学观察发现,服用HS(0.4767~8.5806 g/kg)长达3个月对肝、心、肾基本无毒性。

Objective:To observe the toxicity of the water extract of the Mongolian medicinal herb Carthamus tinctorius L.(HS)to the liver,heart,and kidney.Methods:Rats were intragastrically administrated with low-,medium-,and high-dose HS water extracts once a day for 3 months.Blood was collected from the tail once every 14 days.The serum levels of glutamic-pyruvic transaminase(GPT),glutamic-oxalacetic transaminase(GOT),triglycerides(TG),lactic dehydrogenase(LDH),creatine kinase(CK),urea,and creatinine(Cr)were determined.Three months later,the rats were sacrificed and the liver,heart,lung,kidney,spleen,stomach,intestine,and brain were collected.Hematoxylin-eosin(HE)staining was employed for the observation of pathological changes.Results:The serum levels of GPT,GOT,TG,LDH,CK,Urea,and Cr and the tissue sections showed that the three months of treatment with low-,medium-,and high-dose HS water extracts basically did not cause injuries in the liver,heart or kidney.Conclusion:The administration with HS water extract within 0.4767~8.5806 g/kg for three months has no toxic or side effect on the liver,heart,and kidney.