基于无机元素、有效成分银柴胡产地特征及关联分析

Origin Characteristics and Correlation Analysis of Stellariae Radix Based on Inorganic Elements and Effective Components

目的比较不同产地银柴胡和土壤无机元素及药材有效成分特征,分析土壤与药材无机元素和有效成分的相关性。方法收集来自9个产地银柴胡药材和生境土壤样品,采用ICP-MS技术测定样品16种无机元素含量,绘制无机元素指纹图谱;采用紫外可见分光光度法测定银柴胡总甾醇和总黄酮含量,并对土壤与药材无机元素和总甾醇、总黄酮含量进行Pearson相关性分析。结果不同产地银柴胡药材和土壤具有相对一致的无机元素特征,但因无机元素不同表现出不同的特征差异;药材总甾醇和总黄酮含量亦因产地不同存在较大差异;银柴胡药材无机元素间及其与土壤无机元素存在不同水平的相关性,药材中S元素与总甾醇含量呈显著负相关性,土壤和药材中Mg元素以及土壤中Ca元素与药材总黄酮含量呈显著负相关性,药材中As元素与药材总黄酮含量呈显著正相关性。结论银柴胡及生境土壤的无机元素,表现出不同的协同或拮抗作用,并与药材有效成分表现出一定的相互作用关系,研究结果为银柴胡道地产区建设和科学种植提供了一定的参考。

OBJECTIVE To compare the features of effective components in Stellariae Radix,soil inorganic elements and Stellariae Radix from different habitat,and to analyze the correlation between soil and inorganic elements and effective components of medicinal materials.METHODS The habitat soil samples of Stellariae Radix from 9 habitats were collected,the contents of 16 inorganic elements were determined by ICP-MS.The fingerprint map of inorganic elements was drawn.The contents of total sterols and total flavonoids were determined by UV-vis spectrophotometry,and the Pearson correlation analysis was carried out between the contents of inorganic elements and total sterols and total flavonoids in soil and medicinal materials.RESULTS The medicinal materials of Stellariae Radix and soil from different areas had relatively consistent characteristics of inorganic elements,but exhibited different characteristics due to different inorganic elements,and the contents of total sterols and total flavonoids were also different from different areas.Correlation analysis showed that there were different levels of correlation between inorganic elements and soil inorganic elements,there was a significant negative correlation between S element and total sterol content,there was a significant negative correlation between soil and medicinal material Mg element and soil Ca element and total flavonoids content,and there was a significant positive correlation between As element and total flavonoids content.CONCLUSION The inorganic elements of Stellariae Radix and habitat soil show different synergistic or antagonistic effects,and show a certain interaction with the effective components of medicinal materials.The results provide a reference for the construction and scientific planting of Stellariae Radix.