液相色谱-串联质谱法检测食品中芬太尼类新精神活性物质

Determination of fentanyl new psychoactive substances in food by liquid chromatography-tandem mass spectrometry

建立液相色谱-串联质谱(LC-MS/MS)快速检测食品中芬太尼类新精神活性物质的方法。样品经乙腈加适量水连续振荡提取,加无水乙酸钠促进电离,用无水硫酸镁吸附水分。采用0.1%甲酸水-乙腈作为流动相进行梯度洗脱,质谱(ESI+)采用多反应监测模式(MRM)对芬太尼类新精神活性物质的定量离子和定性离子进行监测。该方法可在6 min内完成7种目标化合物的检测。7种芬太尼类新精神活性物质在1倍、2倍和10倍定量限添加水平的回收率为86.6%~100.4%,相对标准偏差小于5.0%(n=6),方法定量限为1~5μg/kg。该方法快速、准确、灵敏,适合测定识别食品中芬太尼类新精神活性物质。

A rapid method for the identification and detemintation of fentanyl new psychoactive substances in food was established by liquid chromatography-tandem mass spectrometry.The samples were extracted by acetonitrile and an appropriate amount of water by continuous shaking,anhydrous sodium acetate was added to promote ionization,and anhydrous magnesium sulfate was used to adsorb water.0.1%formic acid water(A)and acetonitrile(B)were used as mobile phases for gradient elution.The quantitative and qualitative ions of fentanyl new psychoactive substances were monitored by mass spectrometry(ESI+)using multiple response monitoring mode(MRM).This method could be used to analyze 7 target compounds within 6 min.The recoveries of 7 new psychoactive substances of fentanyl were 86.6%-100.4%at 1,2 and 10 times limit of quantification levels,the relative standard deviations were less than 5.0%(n=6),and the limit of quantification was 1-5μg/kg.The method is rapid,accurate and sensitive,and suitable for the determination and identification of fentanyl new psychoactive substances in food.