5株植物内生真菌生物转化柠檬烯和月桂烯产物分析

Analysis of Biotransformation Products of Limonene and Myrcene by Five Plant Endophytic Fungi

为了筛选具有转化利用单萜类化合物能力的微生物菌株,以5株植物内生真菌为生物催化剂,(+)-柠檬烯、(-)-柠檬烯、月桂烯为生物转化底物,结合气相色谱-质谱联用技术考察生物转化产物.结果表明:5株植物内生真菌具有生物转化(+)-柠檬烯、(-)-柠檬烯、月桂烯的能力;当底物为(+)-柠檬烯时,主要产物均为柠檬烯-1,2-二醇,其中,菌株Diaporthe Nitschke PS10的产量最高,达到4.57 g·L-1;当底物为(-)-柠檬烯时,主要产物为柠檬烯-1,2-二醇,产量为0.81~2.04 g·L-1;当底物为月桂烯时,转化产物有环氧罗勒烯、氧化芳樟醇、柠檬烯-1,2-二醇等化合物;植物内生真菌是一种很有发展潜力的生物催化剂.

In order to screen microbial strains with the ability to transform and utilize monoterpene compounds,five plant endophytic fungi were used as biocatalysts,(+)-limonene,(-)-limonene and myrcene were used as biotransformation substrates,and the biotransformation products were investigated by Gas Chromatography-Mass Spectrometry.The results show that five plant endophytic fungi possess the ability to biotransform(+)-limonene,(-)-limonene and myrcene.When the substrate is(+)-limonene,the main product is limonene-1,2-diol,and the highest yield is 4.57 g·L-1 when the strain is Diaporthe Nitschke PS10.When the substrate is(-)-limonene,the main product is also limonene-1,2-diol,and the yield is 0.81 to 2.04 g·L-1.When the substrate is myrcene,the conversion products include myroxyde,linalool oxide,limonene-1,2-diol and other compounds.It is indicated that plant endophytic fungi are potential biocatalysts.