番茄GT-1基因组织表达模式及非生物胁迫、植物生长调节剂响应分析

Expressions and Responses to Abiotic Stresses and Plant Growth Regulator of Tomato GT-1

[目的]完善番茄GT-1亚家族基因的相关功能信息,为进一步研究Trihelix转录因子调控植物生长发育过程、提高植物非生物胁迫的抗性能力提供参考。[方法]利用生物信息学方法对GT-1基因进行生物进化分析,利用RT-PCR技术鉴定GT-1基因对非生物胁迫和植物生长调节剂的响应。[结果](1)番茄中包含3个GT-1基因,即SlGT-21、SlGT-24和SlGT-35,进化分析表明番茄GT-1基因存在功能分化。(2)表达模式分析发现,3个基因于番茄所有组织中均表达,特别是果实发育阶段,推测SlGT-21、SlGT-35有部分类似功能。(3)3个GT-1基因受干旱抑制,但SlGT-24受抑制更明显;3个基因均响应盐胁迫,SlGT-21、SlGT-35基因被较明显抑制。(4)3个基因受植物生长调节剂GA(赤霉素)、 EBR(表油菜素内酯)、 MeJA(茉莉酸甲酯)抑制,但SlGT-24、 SlGT-35受ABA(脱落酸)诱导,而SlGT-24还受ACC(1-氨基环丙烷羧酸)诱导。[结论]番茄GT-1亚家族3个基因受盐、干旱的调控,且对植物生长调节剂的响应明显。该研究为深入探究GT-1亚家族成员的生物功能提供了重要参考。

【Objective】Genetic information and functions of trihelix factors in GT-1 critical to the growth,development,and resistance to abiotic stresses and plant growth regulator of tomato plants were investigated.【Method】Bioinformatic techniques were applied to decipher the bio-evolutionary of GT-1 in tomato.RT-PCR was used to determine the expressions and responses to abiotic stresses and plant growth regulator of the gene.【Result】(1)In tomato,GT-1 had 3 subfamily members,i.e.,SlGT-21,SlGT-24,and SlGT-35.The evolutionary analysis indicated that those members differentiated functionally.(2)The 3 subfamily genes expressed in all tested tomato tissues with the highest level in the fruits,especially during the growing stage.It was speculated that SlGT-21 and SlGT-35 might share some common functionalities.(3)All 3 genes,particularly SlGT-24,could be suppressed by dehydration,and SlGT-21 and SlGT-35 more severely affected by salt stress.(4)The expressions of these genes were inhibited by GA(Gibberellin),EBR(Epihomobrassinolide),and MeJA(Methyl jasmonate);but those of SlGT-24 induced by ABA(Abscisic acid)and ACC(1-Aminocyclopropanecarboxylic acid),and SlGT-35 by ABA.【Conclusion】Three GT-1 subfamily genes of tomato plants could be regulated by salt and/or dehydration stresses and were sensitive to hormonal stimulations.