摘要
In this study,we investigated the preferable explant types and sterilization method for tissue culture in Chinese chestnut,in order to provide a technical support for the asexual reproduction of Chinese chestnut.The base,middle and apex parts of annual shoots with buds in Chinese chestnut were sampled and cut to 2-3 cm stem segments each with one bud,then sterilized orderly with different duration in 2%NaClO plus 0.1%HgCl_(2)solutions.The results indicated that the duration of 2%NaClO for 20 min+0.1%HgCl_(2)for 15 min exerted an effective disinfection property on the middle parts of annual shoots with buds,and achieved a contamination rate under 5%and a survival rate over 90%.
基金
Supported by The Sub-project of Hebei Collaborative Innovation Center of Chestnut Industry(202202)
The Sub-project of Research and Development Center of Horticultural Crop Breeding Application Technology of Hebei University(YF201404)
Chestnut Science and Technology Backyard of Hebei Qinglong
China Rural Special Technology Association。
