荷叶总生物碱提取工艺优化及生物活性研究

Studies on Optimization of Extraction Process and Bioactivity of Total Alkaloid from Folium Nelumbinis

为优化荷叶总生物碱提取工艺。在考察提取温度、提取时间、pH、料液比、乙醇体积分数5个单因素对荷叶总生物碱提取率影响的基础上,通过Box-Behnken响应面法进一步优化荷叶总生物碱提取工艺。以DPPH和羟基清除率评价荷叶总生物碱抗氧化能力。结果表明最优提取工艺参数为提取温度50℃、提取时间40min、料液比1∶30、乙醇体积分数70%、pH 4.0。在此条件下,荷叶总生物碱的提取率可达0.9195%。抗氧化活性实验表明荷叶生物碱浓度为1 mg/mL时,DPPH和羟基自由基清除能力分别为70%和62%。本研究优化了荷叶总生物碱的提取工艺,初步分析了其抗氧化活性,为荷叶的深入研究和开发提供了一定的理论依据。

Objectiveto optimize the extraction process of total alkaloid from Folium nelumbinis On the basis of examining the effects of extraction temperature,extraction time,pH,solid-liquid ratio,and ethanol volume fraction on the extraction rate of total alkaloids from Folium nelumbinis,the extraction process of total alkaloids from lotus leaf was further optimized by Box-Behnken response surface methodology.Its antioxidant was evaluated by DPPH and hydroxyl scavenging rate.The results showed that the optimal extraction process parameters were extraction temperature 50℃,extraction time 40 min,solid-liquid ratio 1∶30,ethanol volume fraction 70%,pH 4.0.Under this conditions,the extraction rate of total alkaloid from Folium nelumbiniswas 0.9195%.Antioxidant activity experiments showed that when the concentration of lotus leaf alkaloids was 1 mg/mL,the scavenging abilities of DPPH and hydroxyl radicals were 70%and 62%,respectively.In this study,the extraction process of total alkaloids from Folium nelumbinis was optimized,and its antioxidant activity was preliminarily analyzed,which provided a certain theoretical basis for the in-depth research and development of Folium nelumbinis.