lncRNA LINC00978对甲状腺乳头状癌细胞的影响及其可能机制

Effects of LncRNA LINC00978 on the Papillary Thyroid Carcinoma Cells and its Possible Mechanism

目的探讨长链非编码RNA(long non-coding RNA,lncRNA)LINC00978对甲状腺乳头状癌增殖和侵袭的影响及其与MAPK/ERK信号通路的关系。方法选取TPC-1细胞构建沉默LINC00978细胞模型,实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,qRT-PCR)检测si-RNA干扰LINC00978的效果。分别转染si-RNA、si-NC、PBS作为转染组、对照组、空白组。CCK-8法分别检测各组细胞24、48、72、96h增殖能力;划痕实验检测各组细胞迁移能力;Transwell实验检测各组细胞侵袭能力;Western blot法检测MAPK/ERK信号通路相关蛋白表达情况。结果qRT-PCR检测结果显示,转染si-RNA后LINC00978的表达量明显减少(P<0.01)。转染组在转染24、48、72、96h的细胞增殖能力显著降低,差异均有统计学意义(P<0.01)。转染组48h的细胞迁移能力及侵袭能力明显较对照组及空白组低,差异均有统计学意义(P<0.01)。转染组P-ERK1/2蛋白的表达量较其他两组降低(P<0.01),各组间ERK1/2蛋白的表达量比较,差异无统计学意义(P>0.05)。结论lncRNA LINC00978促进甲状腺乳头状癌的增殖、迁移及侵袭,其机制可能跟调控MAPK/ERK信号通路有关。

Objective To investigate the effect of long non-coding RNA(lncRNA)LINC00978 on the proliferation and invasion of papillary thyroid carcinoma and its relationship with the MAPK/ERK signalling pathway.Methods The TPC-1 cells were selected to construct the silencing cell model of LINC00978,and the effect of siRNA interference with LINC00978 was detected by real-time quantitative polymerase chain reaction(qRT-PCR).The transfected si-RNA,si-NC,and PBS were used as the transfection group,control group and blank group.The proliferation ability of cells in each group at 24h,48h,72h and 96h was detected by CCK-8 assay;the cell migration ability of each group was detected by scratch healing assay;the invasion ability of each group was detected by Transwell assay;MAPK/ERK signalling pathway-related proteins expression was detected by Western blot method.Results The results of qRT-PCR showed that the expression of LINC00978 was significantly decreased after transfection with si-RNA(P<0.01).In the transfection group,the cell proliferation ability was significantly decreased at 24h,48h,72h and 96h after transfection,and the differences were statistically significant(P<0.01).The cell migration ability and invasion ability in the transfection group at 48h was significantly lower than those of the control group and the blank group,and the differences were statistically significant(P<0.01).The expression level of P-ERK1/2 protein in the transfection group was lower than that in the other two groups(P<0.01),and there were no significant differences in the expression of ERK1/2 protein among the groups(P>0.05).Conclusion LncRNA LINC00978 can promotes the proliferation,migration and invasion of papillary thyroid carcinoma,and its mechanism may be related to the regulation of the MAPK/ERK signaling pathway.