穴位埋线对肺虚型变应性鼻炎大鼠的行为学影响

Effect of catgut embedding at acupoints on the behavior of rats with allergic rhinitis in the pattern of Lung deficiency

目的观察穴位埋线对肺虚型变应性鼻炎大鼠行为学的影响。方法45只雌性SD大鼠随机分为埋线组(A组)、模型组(B组)、空白组(C组),每组15只。先用“硫磺艾条烟熏法”在A、B两组制备肺虚模型,再用“卵清蛋白致敏法”在A、B两组动物进一步建立变应性鼻炎模型。然后,行相应穴位埋线3周,比较观察期间三组大鼠的行为学变化。结果造模后A、B两组动物均出现典型症状表现。造模动物呼吸频率增幅PAB=0.084,PAC<0.001,PBC<0.001,体重质量增幅PAB=0.205,PAC<0.001,PBC<0.001。行为学评分阶段性显示,除基础致敏结束后PAB=0.544和强化致敏结束后PAB=0.442以外,其余各阶段两两比较为PAB<0.001,PAC<0.001,PBC<0.001;行为学评分(终点因子),喷嚏、流涕、抓鼻三因子的两两比较分别为PAB<0.001,PAC<0.001,PBC<0.001。结论穴位埋线3周,对肺虚型变应性鼻炎大鼠显示了明显疗效,但黏膜组织变态反应性炎症仍在持续,有必要延长埋线时间以巩固疗效。

Objective To observe the effects of catgut acupoint embedding on the behavior of rats with allergic rhinitis(AR)in the pattern of Lung deficiency.Methods Forty-five female SD rats were randomly divided into catgut embedding group(GA),model group(GB)and blank group(GC),with 15 animals in each.Firstly,Lung deficiency model was prepared by sulfur moxa stick smoking method,and then allergic rhinitis model was established by ovalbumin sensitization method among the animals of GA and GB respectively.Thereafter,all rats in 3 groups were treated by catgut embedding group for 3 weeks to observe the effects of catgut acupoint embedding on their behavior.Results Following modeling,rats in GA and GB were seen with typical symptom manifestations of Lung deficiency and AR.When compared with that before modelling,the increase in respiratory rate after modelling being PAB=0.084,PAC<0.001,PBC<0.001 and the weight gain before and after modeling being PAB=0.205,PAC<0.001,PBC<0.001.While PAB=0.544 by the end of basic sensitization stage and PAB=0.442 by the end of intensive sensitization stage,the statistical analysis showed the pairwise comparison being PAB<0.001,PAC<0.001 and PBC<0.001 by the end of other stages as shown in the staging evaluation.Furthermore,the comparative analysis on the behavioral changes showed PAB<0.001,PAC<0.001,PBC<0.001 among these rats in different groups,mainly manifesting in the 3 main symptoms like sneezing,running nose and scratching nose.Conclusion Catgut embedding at acupoint for 3 weeks holds obvious therapeutic effect on rats with allergic rhinitis in the pattern of Lung deficiency,but the allergic inflammation within the mucosal tissues might continue for some duration.It is necessary to prolong the period of catgut embedding at acupoints to consolidate the therapeutic effect.