解钾JX-10菌的筛选及解钾工艺

Screening of Potassium Dissolving JX-10 Bacteria and Research on the Optimization of Potassium-dissolving

本文从一钾长石矿区土壤中分离、筛选得到了6株高效解钾细菌。其中命名为JX-10的菌株分离于矿区茼蒿根系土壤,经基因测序鉴定为Bacillus sp.,与KT981886菌相似度高到99.72%。同时就培养时间、温度及转速等解钾工艺条件进行了探讨。实验结果表明,JX-10菌株具有分解钾长石矿物的能力。其较佳解钾工艺条件为:培养温度28℃、时间10 d,转速160 r/min,培养基pH值为5.0,钾长石浓度2 g/L,粒度0.03mm,接种量25%,硫酸铵浓度0.2 g/L。在较佳工艺条件下,溶液中可溶性钾离子含量可达23.32 mg/L,浸出率为8.36%。

In this study,six strains with potassium-dissolving ability were screened from the soils collected from a K-feldspar mining area.One of them,which named JX-10 strain was identified as a kind of bacterium,by 16 S rRNA gene sequencing.Simultaneously,the optimization of potassium-dissolving were also discussed.The results show that,the JX-10 strain had an obvious dissolution effect on K-feldspar.The optimum conditions for the JX-10 strain to remove potassium from K-feldspar were as follows:cultured at 28℃for 10 days,pH value of the culture medium of 5.0,60 mL medium in a 250 mL conical flask,and 160 r/min shaking speed on a rotary shaker.The K-feldspar concentration and granularity,inoculation volume,ammonium sulfate dose were 2 g/L,0.03 mm,25%,0.2 g/L,respectively.Under the above conditions,the highest the potassium content and corrosion efficiency reach to 23.32 mg/L and 8.36%,respectively.