4种金花茶叶绿体基因组比较分析

Comparative analysis of chloroplast genome of 4 kinds of yellow Camellia

利用全基因组重测序数据组装得到了夏石金花茶等4种金花茶的叶绿体全基因组序列,并进行了注释和比较分析.结果表明,4种金花茶植物叶绿体基因组序列高度相似,约为156657~157046 bp,均预测注释134个基因,包含89个蛋白编码基因、8个rRNA和37个tRNA;金花茶植物的叶绿体基因组在结构和进化上具有保守性,它们具有相似的密码子偏好性且均未发生大面积的倒位和基因重排;通过叶绿体基因组的比较分析和核苷酸多态性分析发掘了rps16和ycf1等高变异片段,这些片段可作为金花茶植物的分子标记;基于金花茶叶绿体基因组数据构建的系统进化树具有较高的支持度,说明金花茶叶绿体基因组序列的公布对其系统发育的研究具有重要意义.

Chloroplast genomes of 4 species of yellow Camellia including Camellia xiashiensis were assembled from whole-genome resequencing data for annotation and comparative analysis.The results showed that the 4 species of yellow Camellia had highly similar chloroplast genome sequences with lengths ranging from 156657-157046 bp,which were predicted to annotate 134 genes,including 89 protein-coding genes,8 rRNAs and 37 tRNAs.Comparative analysis revealed that the chloroplast genomes of yellow Camellia were highly conservative in structure and evolution,and they had similar codon preferences without extensive gene inversion and rearrangement.Polymorphism analysis revealed highly variable fragments such as rps16 and ycf1 in the chloroplast genomes of yellow Camellia,which can be applied as molecular markers.The phylogenetic tree constructed based on the chloroplast genome data had a high degree of accuracy,indicating that the publication of the chloroplast genome was important for the phylogenic study of yellow Camellia.