暗纹东方鲀鱼皮胶原蛋白的提取工艺优化及其功能特性

Optimization of Preparation Process of Collagen from Takifugu Obscurus Skin and Its Functional Properties

暗纹东方鲀鱼皮胶原蛋白含量丰富,为增加其附加值,对鱼皮胶原蛋白进行制取和功能特性研究。实验以暗纹东方鲀鱼皮为研究对象,探究胃蛋白酶加酶量、酶解时间、辅助超声功率和超声时间对胶原蛋白提取率的影响;采用葡聚糖凝胶柱层析法对提取、盐析除杂的胶原蛋白进行进一步纯化,探究纯化的胶原蛋白对酪氨酸酶及透明酸酶的抑制活性。结果表明,胃蛋白酶加酶量4.2%、酶解时间38 h、超声功率320 W,超声时间9 min,胶原蛋白提取率最高71.6%±2.7%;在浓度为0.8 mg/mL,pH为5.5时,暗纹东方鲀鱼皮胶原蛋白酪氨酸酶抑制率为85%,透明质酸酶的抑制效果高达90%,其效果优于市售鱼皮胶原蛋白,具有更广泛的应用价值。

The skin of Takifugu obscurus is high in collagen.There is a growing trend to study the preparation method and functional properties of fish skin collagen due to the additive values.This research aimed to optimize the collagen extraction processing from the skin of dark-striped pufferfish including the effects of pepsin enzyme addition,enzymatic hydrolysis time,ultrasonic power and ultrasonic time.The dextran gel column chromatography method was used to separate and identify the extracted and salt-desorized collagen.The inhibitory activity of extracted collagen on tyrosinase and hyaluronase was studied.Results showed that,the best extraction rate of collagen was 71.6%±2.7%at pepsin 4.2%,enzymatic hydrolysis time 38 h,ultrasonic power 320 W,and the ultrasonic time 9 mins.The inhibition rate of tyrosinase and hyaluronidase was 85%and 90%respectively,at concentration of Takifugu obscurus skin collagen was 0.8 mg/mL and the pH was 5.5,which performed better than the commercial fish skin collagen.This study indicated that the skin collagen of Takifugu obscurus had the great potential in whitening and anti-inflammatory application,which could be further applied in the field of cosmetics.