不同盐胁迫对紫金牛生长以及生理的影响

Effects of different salt tress on growth and physiology of Ardisia japonica

紫金牛为紫金牛科低矮观赏性小灌木,耐荫性强,在园林广泛用作地被。研究其在不同盐浓度土壤中的生长及适应机量对于推广其在盐碱地上进行园林绿化具有重要意义。以三年生盆栽紫金牛苗为试验材料,红黄壤-椰糠-黑土(体积比为1:1:1)为基质,研究了不同盐对其的影响。设置七种处理,CK、A1(0.30%NaCl)、A2(0.60%NaCl)、B1(0.30%Na_(2)CO_(3))、B2(0.60%Na_(2)CO_(3))、C1(0.15%NaCl+0.15%Na_(2)CO_(3))、C2(0.30%NaCl+0.30%Na_(2)CO_(3))。对植株株高、MDA、可溶性蛋白、叶绿素含量、叶绿素荧光参数等进行了测定。随着胁迫程度的增加,紫金牛的株高增量、根茎含水量逐渐降低;同时,细胞膜受到伤害,细胞膜透性发生变化,作为膜脂过氧化作用的主要产物MDA随之增加;紫金牛的可溶性蛋白先升高后降低,这说明胁迫达到一定程度后,可溶性蛋白降解,低浓度的盐胁迫初期会有一定的促进作用,所以会呈现先下降再升高的趋势;胁迫期间Fv′/Fm′、ETR、Fv/Fm值下降。结果表明,紫金牛具有一定的耐盐性,紫金牛可在含轻度中性盐和混合盐土地区栽植,不适宜栽植于碱性盐地区。

Ardisia japonica is a low ornamental shrub belong to myrsinacea family.It is widely used as a ground cover in gardens because of strong shade tolerance.It is of great significance to study the growth and adaptability of A.japonica in soils with different salt concentration for popularizing its landscaping in saline-alkali soil.The effects of different salts on triennial potted A.japonica seedlings were studied,with red-yellow soil,soil-coconut bran,and black soil(with a volume ratio:1:1:1)as the substrate.Seven treatments were set,including CK,A1(0.30%NaCl),A2(0.60%NaCl),B1(0.30%Na_(2)CO_(3)),B2(0.60%Na_(2)CO_(3)),C1(0.15%NaCl+0.15%Na_(2)CO_(3))and C2(0.30%NaCl+0.30%Na_(2)CO_(3)).The plant height,MDA,soluble protein,chlorophyll content and chlorophyll fluorescence parameters were measured.With the increasing of stress degree,the plant height increment and rhizome water content of A.japonica gradually decreased.Meanwhile,the cell membrane was damaged,and the permeability changed,and MDA,the main product of membrane lipid peroxidation increased accordingly.The soluble protein of A.japonica first increased and then decreased,which indicated that the protein would degrade when the stress reached a certain degree,low concentration of salt promoted promoted effect at the early stage of the stress.During the stress,the value of Fv´/Fm´,ETR,Fv/Fm decreased.The results showed that A.japonica had a certain salt tolerance,and A.japonica could be planted in areas containing mild neutral salt and mixed saline soil,which was not suitable for planting in alkaline salt areas.