摘要
The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has wreaked havoc around the globe,with no end in sight.The rapid emergence of viral mutants,marked by rapid transmission and effective immune evasion,has also posed unprecedented challenges for vaccine development,not least in its speed,mass production,and distribution.Here we report a versatile“plug-and-display”strategy for creating protein vaccines,including those against malaria parasites and SARS-CoV-2,through the combined use of the intrinsically disordered protein ligase SpyStapler and computationally designed viral-like particles.The resulting protein nanoparticles harboring multiple antigens induce potent neutralizing antibody responses in mice,substantially stronger than those induced by the corresponding free antigens.This modular vaccine design enabled by SpyStapler furnishes us with a new weapon for combatting infectious diseases.
基金
the Ministry of Science and Technology to F.S.(No.2020YFA0908100)
from the National Natural Science Foundation of China Excellent Young Scientists Fund to F.S.(No.22122707)
from National Natural Science Foundation of China Youth fund to S.Z.K.(No.21907003)
from the National Natural Science Foundation of China from Guangdong Natural Science Foundation to F.S.(No.2019A1515011691)
S.Z.K.(No.2020A1515011429)
from the Science,Technology,and Innovation Commission of Shenzhen Municipality to F.S.(Shenzhen-Hong Kong-Macao S&T Program(Category C)#SGDX2020110309460101
Key Research Program#JCYJ20200109141241950
Basic Research Program#JCYJ20190813094601656)
from the Research Grants Council of Hong Kong SAR Government to F.S.(General Research Fund#16103519 and#16103421
Theme-based Research Scheme#T13-602/21-N)
from the State Key Laboratory of Molecular Neuroscience,HKUST,China,and in part from the Innovation and Technology Commission(ITCPD/17-9)are acknowledged.
