梧桐子化学成分及其体外促凝血活性

Chemical constituents from the seeds of Firmiana simplex and their procoagulant activities in vitro

目的研究梧桐Firmiana simplex(L.)W.Wight种子的化学成分及其体外促凝血活性。方法梧桐子85%乙醇提取物采用大孔吸附树脂、硅胶、MCI、ODS、Sephadex LH-20、Toyopearl HW-40F凝胶柱色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用凝血四项试剂盒测定其体外促凝血活性。结果从中分离得到18个化合物。化合物1~5、7~8、10~11、13均有促凝血作用。结论化合物2~7、9~11、13~17为首次从梧桐属植物中分离得到,化合物12为首次从该植物中分离得到。化合物2~3有很好促凝血活性,效果与云南白药相当,化合物1、5、8、10有良好促凝血活性,化合物4、7、11、13有一定促凝血活性。

AIM To study the chemical constituents from the seeds of Firmiana simplex(L.)W.Wight and their procoagulant activities in vitro.METHODS The 85%ethanol extract from the seeds of F.simplex was isolated and purified by macroporous adsorption resin,silica gel,MCI,ODS,Sephadex LH-20 and Toyopearl HW-40F gel column chromatography,then the structures of obtained compounds were identified by physical and chemical properties and spectral data.Their procoagulant activities were evaluated the four-item coagulation kit in vitro.RESULTS Eighteen compounds were isolated and identified.Compounds 1-5,7-8,10-11,13 demonstrated procoagulant effects.CONCLUSION Compounds 2-7,9-11,13-17 were isolated from genus Firmiana for the first time.Compound 12 was isolated from this plant for the first time.Compounds 2-3 show excellent procoagulant activities,which were comparable to Yunnan Baiyao,compounds 1,5,8,10 show good procoagulant activities,and compounds 4,7,11,13 show certain procoagulant activities.