基于DCI的近红外探针和微信小程序的便携式荧光传感平台用于ONOO^(-)的分析检测

Detection of ONOO^(-)Using a Portable Fluorescence Sensing Platform Based on Pyronitrile Near-infrared Probe and WeChat Applet

该研究设计并合成了一种基于吡喃腈类(DCI)荧光衍生物的近红外探针1a用于特异性检测ONOO-。该探针本身具有橙色荧光,经ONOO^(-)刺激后,探针的苯硼酸频哪醇酯结构脱落,母体分子的内电荷转移(ICT)效应恢复,使得荧光发射增强,荧光颜色由橙色变为红色。此外,探针1a具有良好的选择性和抗干扰能力,检出限低至97 nmol/L。同时,通过自主开发的微信小程序“色度采集(Colorimetric acquisition)”配合探针1a评估色度与ONOO^(-)浓度的相关性,搭建了可对外源性ONOO^(-)检测效果进行智能评估的简易检测平台,该检测平台有望成为便携智能检测环境中有害物质的简易分析工具。该文利用近红外染料开发的探针1a可用于ONOO-的检测,为智能化检测有害物质提供了新的参考。

A portable fluorescence sensing platform based on pyronitrile(DCI)near-infrared probe 1a and WeChat applet was constructed for the detection of ONOO^(-).The near-infrared probe 1a was firstly designed and synthesized based on a DCI fluorescent derivative,which was used for specific detection of ONOO^(-).The probe itself possess orange fluorescence,and the probe’s phenylboronic acid pinacol ester structure sheds after stimulation with nitrite ONOO^(-),then the parent intramolecu⁃lar charge transfer(ICT)effect is restored,resulting in enhanced fluorescence emission and a color change of the probe’s fluorescence from orange to red after activation.Meanwhile,probe 1a exhibit⁃ed excellent selectivity and anti-interference ability,the ultra-low detection limit reached 97 nmol/L.In addition,a simple detection platform based on a WeChat mini program“Colorimetric acquisition”was established,relying on the correlation between luminous chrominance of probe and ONOO^(-)fluo⁃rescence response to probe,which could be used to intelligently evaluate the detection effect of exog⁃enous ONOO^(-).This detection platform has a potential to become a simple analysis tool for portable and intelligent detection of hazardous substances in the environment.In conclusion,the NIR dyebased probe 1a developed in this paper was applied to the detection of ONOO^(-),providing a new reference for the intelligent detection of hazardous substances.