真核翻译起始因子4A在上皮性卵巢癌中的表达及与患者预后的关系

Expression of eukaryotic translation initiation factor 4A in epithelial ovarian carcinoma and its relationship with prognosis of patients

目的探讨真核翻译起始因子4A(eIF4A)蛋白在不同卵巢组织中的表达及其与上皮性卵巢癌(EOC)患者预后的关系。方法取123例EOC组织、30例交界性卵巢癌组织、15例卵巢良性病变组织和20例正常卵巢上皮组织,采用免疫组化法检测eIF4A蛋白的表达情况,并比较不同临床特征EOC患者EOC组织中eIF4A蛋白的表达情况,荧光原位杂交(FISH)检测eIF4A基因扩增情况。EOC患者预后的影响因素采用Cox比例风险回归模型分析。结果eIF4A蛋白高表达率在正常卵巢上皮组织、卵巢良性病变组织、交界性卵巢癌组织及EOC组织中依次升高,差异有统计学意义(P﹤0.05)。123例EOC患者EOC组织中,eIF4A蛋白高表达93例,低表达30例,FIGO分期为Ⅲ~Ⅳ期、组织学分级为G3级、有淋巴结转移、有腹腔积液EOC患者EOC组织中eIF4A蛋白高表达率分别高于临床分期为Ⅰ~Ⅱ期、组织学分级为G1~2级、无淋巴结转移、无腹腔积液的患者,差异均有统计学意义(P﹤0.05)。eIF4A高表达患者的5年总生存率为28.8%,明显低于低表达患者的45.8%(P﹤0.01)。Cox比例风险回归模型分析结果显示,临床分期为Ⅲ~Ⅳ期、组织学分级为G3级、术后病变残余均是EOC患者预后的独立危险因素(P﹤0.05)。结论eIF4A在EOC的发生、发展中发挥重要作用,其高表达与患者的不良预后密切相关。

Objective Explore the expression of eukaryotic translation initiation factor 4A(eIF4A)protein in different ovarian tissues and its relationship with the prognosis of epithelial ovarian carcinoma(EOC)patients.Method A total of 123 cases of EOC tissues,30 cases of borderline epithelial neoplasia tissues,15 cases of benign epithelial neoplasia tissues and 20 cases of normal ovarian tissues were collected,the expressions of eIF4A protein were detected by immunohistochemistry,and the expressions of eIF4A in EOC tissues of patients with different clinical features were compared.The amplification of eIF4A gene was detected by fluorescence in situ hybridization(FISH).The factors influencing prognosis of EOC patients were analyzed by using Cox proportional-hazards model.Result The high expression rate of eIF4A protein in normal ovarian tissues,benign epithelial neoplasia tissues,borderline epithelial neoplasia tissues and EOC tissues increased in sequences(P<0.05).There were 93 cases of high expression and 30 low expression of eIF4A protein in 123 cases of EOC tissues.The high expression rate of eIF4A protein in EOC tissues of patients with FIGO staging of stage III-IV,histological grading of G3,lymph node metastasis,and peritoneal effusion were higher than those with clinical staging of stage I-II,histological grading of G1-2,no lymph node metastasis,and no peritoneal effusion,respectively,with differences were statistically significant(P<0.05).The overall 5-year survival rate of eIF4A high expression patients was 28.8%,lower than 45.8%of low expression patients(P<0.01).The results of Cox proportional risk regression model analysis showed that clinical stage III-IV,histological grade G3,and postoperative residual lesions were independent risk factors for the prognosis of EOC patients(P<0.05).Conclusion The eIF4A plays an important role in the oncogenesis and progression of EOC,and the EOC patents with high eIF4A expression might have poor prognosis.