咪鲜胺对子代雄鼠的基因表达影响研究

Research on Alterations of Gene Expression in Offspring Male Mice Affected by Prochloraz

咪鲜胺是一种内分泌干扰物农药,环境残留对人类的健康影响极大。以小鼠为模式动物研究不同剂量咪鲜胺对子代小鼠的生殖系统影响,可以评估其残留对于人类健康的影响。结果发现高浓度组(MD)、低浓度组(MB)和对照组(MA)之间基因表达聚类分析图,显示其具有很好的组间区别性和组内高度重复性,为下一步的分析奠定了基础;后续进一步通过基因功能(GO)和代谢途径(Pathway)分析显示,主要涉及生殖系统有关的功能与途径(如神经肽激素调控信号通路、免疫反应、抗原加工和呈递和前列腺素的生物合成等)。由此可见,咪鲜胺对雄性子代雄性小鼠具有一定的生殖毒理影响。

Prochloraz is an endocrine disruptor pesticide,and its environmental residues have a great impact on human health.Through the study of the effects of different doses of prochloraz on the reproductive system of offspring mice,we can evaluate the effects of its residues on human health.The results showed that the heat map of gene expression cluster analysis of high concentration group(MD),low concentration group(MB)and control group(MA)had good inter-group difference and high intra-group repeatability,which laid a foundation for further analysis.The subsequent analysis of gene ontology(GO)and metabolic pathway showed that prochloraz mainly involves the functions and pathways related to the reproductive system(such as neuropeptide hormone regulatory signal pathway,immune response,antigen processing and presentation,prostaglandin biosynthesis,etc.).In conclusion,it can be seen that prochloraz has a certain reproductive toxicological effect on offspring male mice.