北京市Redondoviridae流行情况初步调查与分析

Preliminary investigation and analysis on the epidemic situation of Redondoviridae in Beijing

目的了解Redondoviridae在北京市流行情况、分析其流行特征。方法收集北京市健康人群及急性呼吸道感染病例,包括流感样病例、住院严重急性呼吸道感染(severe acute respiratory infection,SARI)病例的咽拭子样本,使用实时荧光PCR法进行核酸检测,对Redondoviridae阳性样本通过扩增测序,分析其种别,通过统计分析获得Redondoviridae年龄分布、性别分布及种别分布等流行特征。使用多重PCR法对流感样病例、SARI病例中Redondoviridae阳性样本进行其他常见呼吸道病原体检测,分析Redondoviridae的致病性。结果健康人群、急性呼吸道感染病例Redondoviridae阳性率分别为20.48%(189/923)、11.23%(43/390),差异具有统计学意义(χ^(2)=16.834,Ρ<0.001)。健康人群中男性阳性率高于女性,老年组阳性率高于成年组与未成年组,差异具有统计学意义(χ^(2)=11.061,Ρ<0.05;χ^(2)=10.088,Ρ<0.05)。急性呼吸道感染者中男性阳性率高于女性,但差异无统计学意义,未成年组阳性率低于老年组与成年组,差异具有统计学意义(χ^(2)=25.724,Ρ<0.05)。Redondoviridae阳性样本中Vientovirus占比高于Brisavirus,差异有统计学意义(P<0.05)。呼吸道感染病例咽拭子中有43例Redondoviridae阳性,其中24例未检测到其他病原体。结论Redondoviridae在北京市各年龄组健康人群中广泛存在,在急性呼吸道感染病例中也有发现,其检出阳性率在不同年龄、性别间存在差异。Vientovirus与Brisavirus两种病毒均有检出,Vientovirus占比明显高于Brisavirus。

Objective To understand the epidemic situation of Redondoviridae in Beijing and analyze its epidemiologic characteristics.Methods Pharyngeal swab samples of healthy people and patients with acute respiratory infection in Beijing,including influenza like cases and severe acute respiratory infection(SARI)cases in hospitals were collected.Real time PCR was used to detect the nucleic acid of Redondoviridae.The positive samples were amplified and sequenced to analyze their species.The age and sex distribution of patients and species distribution of Redondoviridae were obtained through statistical analysis.Multiplex PCR was used to detect other common respiratory pathogens in the positive samples of Redondoviridae in influenza like cases and SARI cases,and the pathogenicity of Redondoviridae was analyzed.Results The positive rates of Redondoviridae in healthy people and acute respiratory infection cases were 20.48%(189/923)and 11.23%(43/390),respectively,with a statistically significant difference(P<0.05).The positive rate of male was higher than that of female in the healthy population,and the positive rate of the elderly group was higher than that of the adult group and the underage group,with a statistically significant difference(P<0.05).The positive rate of male patients with acute respiratory tract infection was higher than that of female patients,but there was no significant difference.The proportion of Vientovirus in the positive samples of Redondoviridae was higher than that of Brisavirus,and the difference was statistically significant(P<0.05).Among the throat swabs of respiratory tract infection cases,43 were positive for Redondoviridae,of whom 24 were not detected for other pathogens.Conclusions Redondoviridae widely exists in healthy people of all age groups in Beijing,and is also found in acute respiratory infection cases.The positive rate of Redondoviridae is different in different ages and genders.Both Vientovirus and Brisavirus were detected,and the proportion of Vientovirus was significantly higher than Brisavirus.