数字PCR技术在胃肠道病毒检测中的应用进展

Application of digital PCR in detection of gastrointestinal viruses

胃肠道病毒包括急性胃肠炎病毒和肠道病毒。此类病毒传染性较强,人群普遍易感,仅需数十至数百个病毒颗粒便可引发感染。数字PCR技术(digital polymerase chain reaction,dPCR)具有灵敏度高、抗干扰性强、直接定量的优点,在胃肠道病毒的检测,尤其是低病毒载量样本的检测中展现了特有的优势,是实时荧光定量PCR技术的有益补充。本文对近年dPCR技术在胃肠道病毒检测中的应用进行综述与展望。

Gastrointestinal viruses include acute gastroenteritis virus and enterovirus.These viruses are highly contagious and human populations are generally susceptible to them,and the viruses require only tens to hundreds of virus particles to cause infection.Digital polymerase chain reaction(dPCR)has the advantages of high sensitivity,strong anti-interference and direct quantification.It has shown its uniqueness in the detection of gastrointestinal viruses,especially for samples with low viral loads,which is a beneficial supplement to the real-time PCR technology.This article reviews and looks forward to the application of digital PCR technology in gastrointestinal virus detection.