基于近红外自体荧光技术的甲状旁腺快速识别系统

Rapid Parathyroid Recognition System Based on Near-Infrared Autofluorescence

基于近红外自体荧光技术,设计一种甲状旁腺快速识别系统,该系统对术中快速识别甲状旁腺具有重要价值.设计一种环形可调激发光源和高精度可调LED恒流源,利用近红外光源激发组织荧光同时通过高灵敏度CMOS相机采集组织自体荧光信息,并对采集的荧光图像进行图像处理,从而准确识别甲状旁腺.利用梯度浓度吲哚菁绿(ICG)溶液模拟组织荧光,实验测得荧光强度与ICG浓度成正相关,信噪比与信背比均符合术中辨别需求,验证了本系统应用于不同荧光强度时的灵敏性与准确性.采用本系统对组织仿体进行测试,荧光仿体能够与背景明显区分开.对甲状旁腺及周边组织进行测试,甲状旁腺呈绿色,与周边组织明显区分,初步验证了本系统可用于对甲状旁腺的识别检测.

Based on nearinfrared autofluorescence,a rapid identification system for parathyroid glands during surgery is designed,the system is of great value for rapid identification of parathyroid glands during operation.In this research,a ringshaped adjustable excitation light source and a highprecision adjustable LED constant current source are designed.The nearinfrared light source is used to excite tissue fluorescence,and the tissue autofluorescence information is collected by a highsensitivity CMOS camera.The obtained fluorescence images are processed,accurately identifying parathyroid glands.Simulating tissue fluorescence through gradient concentration of indocyanine green(ICG)solution,the experimentally measured fluorescence intensity is positively correlated with the concentration of ICG,and both the signaltonoise ratio and signaltobackground ratio meet the requirements for intraoperative discrimination,which verifie the sensitivity and accuracy of the proposed system for different fluorescence intensities.Using this system to test tissue phantoms,the fluorescent phantom can be clearly distinguished from the background.The parathyroid gland and surrounding tissues were tested,and the parathyroid gland is green and clearly distinguished from the surrounding tissues,which preliminarily verifies that the proposed system can be used for the identification and detection of parathyroid glands.