深海热液区管状蠕虫Ridgeia piscesae新型C型凝集素的克隆与表达

Cloning and expression of a novel C-type lectin from tube worm Ridgeia piscesae

C型凝集素作为凝集素家族的主要成员之一,在无脊椎动物中有着丰富的多样性,其独特的生物学功能广泛应用于生物、医药等诸多领域。本研究从采集于东北太平洋深海热液区胡安·德富卡洋脊的管状蠕虫Ridgeia piscesae中克隆获得了一种新型C型凝集素基因RPCL。该基因与已报道的凝集素的相似性都很低,并且三维结构预测有异于传统凝集素,提示其可能具有一些特殊的功能性质。进一步的原核表达成功实现了RPCL的异源重组表达,通过透析复性获得了大量可溶性蛋白,为其相关功能性质的研究打下了坚实的基础。

C-type lectin is the main member of the lectin family,which has abundant diversity in invertebrates.Their unique biological functions are widely applied in many fields,such as biology,medicine and so on.In this study,a novel C-type lectin RPCL was cloned from Ridgeia piscesae,a tube worm collected from the deep-sea hydrothermal region at Juan de Fuca Ridge in the northeast Pacific Ocean.The similarity between this gene and the reported lectins is very low,and the predicted 3D structure was quite different from the known lectins,suggesting that it may have some special properties.Furthermore,the heterologous recombinant expression of RPCL was successfully achieved,and a large amount of soluble proteins were obtained through purification and renaturation.It will facilitate the further study on the function of RPCL.