香港巨牡蛎抗副溶血弧菌感染相关microRNA的筛选

Screening of microRNAs related to Vibrio parahaemolyticus infection in Hong Kong oyster(Magallana hongkongensis)

香港巨牡蛎(Magallana hongkongensis,旧称Crassostrea hongkongensis)是我国华南沿海重要的经济贝类,但频发的养殖病害严重制约了香港巨牡蛎养殖业的可持续发展。作为一类重要的调控因子,microRNA(miRNA)能够对其靶基因进行转录后调控从而参与海洋动物各类生命活动过程。本研究利用高通量测序技术建立了副溶血弧菌不同诱导时间下的香港巨牡蛎鳃组织miRNA文库,并通过生物信息学分析方法挖掘与免疫调控有关的miRNA,共获得了453个已知miRNA和222个新miRNA,通过进一步筛选空白对照组/12 h病原注射组、空白对照组/24 h病原注射组、12 h病原注射组/24 h病原注射组特有的miRNA,发现多个在不同比较组间差异表达的或具有高表达水平的miRNA。其中miR-10、miR-23、miR-92、miR-100、miR-125、miR-144、miR-145、miR-146、miR-335、miR-750等已被证实参与了多种海洋动物的免疫调控过程。对差异表达miRNA靶基因的KEGG富集分析结果显示miR-411-5p、miR-4454-5p、novel-m0159-3p、novel-m0187-3p的靶基因涉及免疫相关通路。分析结果表明上述miRNA可能在香港巨牡蛎免疫调控过程中发挥重要作用,有必要对其开展进一步研究。

Hong Kong oyster(Magallana hongkongensis)is an important economic shellfish cultivated in the coastal areas of southern China.However,breeding diseases frequently happen during cultivation,and they have seriously restricted the sustainable development of Hong Kong oyster farming industry.As an important regulatory factor,microRNA(miRNA)participates in various biological processes of marine species through post-transcriptional regulation of their target genes.In this study,we used high-throughput sequencing technology to create miRNA libraries from gill of Hong Kong oyster under different induction time points of Vibrio parahaemolyticus infection and then explored the miRNAs related to immune regulation via bioinformatics analysis.In total,we obtained 453 conserved miRNAs and 222 novel miRNAs.Subsequently,the unique miRNAs among blank control group/12 h pathogen-injection group,blank control group/24 h pathogen-injection group,12 h pathogen-injection group/24 h pathogen-injection group were further screened.As result,a number of differentially expressed or highly expressed miRNAs were found in different comparison groups.Among them,miR-10,miR-23,miR-92,miR-100,miR-125,miR-144,miR-145,miR-146,miR-335 and miR-750 have been verified to involve in the immune regulation processes in a variety of marine animals.KEGG enrichment analyses of target genes of differentially expressed miRNAs showed that the target genes of miR-411-5p,miR-4454-5p,novel-m0159-3p and novel-m0187-3p participated in immune-related pathways.These results indicated that the above-mentioned miRNAs may play an important role in the immune regulation of Hong Kong oysters,Thus,it is necessary to further explore on them.