猪繁殖与呼吸综合征病毒感染Marc-145细胞的长链非编码RNA和mRNA转录组分析

Transcriptome Analysis of Long Non⁃coding RNA and mRNA Profiles in PRRSV⁃infected Marc⁃145 Cells

为了挖掘猪繁殖与呼吸综合征病毒(PRRSV)感染Marc-145细胞后宿主细胞长链非编码RNA(lncRNA)和mRNA表达谱的变化,对PRRSV感染Marc-145细胞的lncRNA和mRNA进行转录组测序和分析。生物信息学分析结果显示,PRRSV感染后Marc-145细胞有39个差异表达lncRNA(DElncRNA)和1320个差异表达mRNA(DEG)。进一步的富集分析结果显示,这些DElncRNA和DEG主要富集在氧化磷酸化信号通路。DElncRNA和DEG的联合分析结果显示,与lncRNA共表达的mRNA主要富集在MAPK信号通路、胰岛素信号通路、神经营养素信号通路、AMPK信号通路、HIF-1信号通路、内质网中的蛋白质加工和碳代谢相关的信号通路,这些通路主要与宿主免疫有关。综上,PRRSV感染导致Marc-145细胞的lncRNA和mRNA表达谱均发生了改变,经生物信息学分析,推测差异表达产物可能在宿主对PRRSV感染的先天免疫反应中发挥重要作用。

In order to dig the changes of lncRNA and mRNA expression profiles in host cells after porcine reproductive and respiratory syndrome virus(PRRSV)infection of Marc⁃145 cells,the lncRNA and mRNA of Marc⁃145 infected with PRRSV were sequenced and analyzed by transcriptome.Bioinformatics analysis showed that 39 differentially expressed lncRNAs(DElncRNAs)and 1320 differentially expressed mRNAs(DEGs)were obtained in Marc⁃145 cells after PRRSV infection.Further enrichment analysis results showed that these DElncRNAs and DEGs were mainly enriched in oxidative phosphorylation signaling pathway.Integration analysis of DElncRNAs and DEGs showed that lncRNA co⁃expressed mRNAs were mainly enriched in MAPK signaling pathway,insulin signaling pathway,neurotrophin signaling pathway,AMPK signaling pathway,HIF⁃1 signaling pathway,protein processing in endoplasmic reticulum and carbon metabolism,which were mainly related to immunity.In conclusion,RRSV infection causes changes in the lncRNA and mRNA expression profiles of Marc⁃145 cells.By bioinformatics analysis,it is speculated that the differential expression products may play an important role in the host’s innate immune response to PRRSV infection.