青蒿琥酯对痤疮丙酸杆菌诱导的人皮脂腺细胞促炎症因子表达的影响

The effect of artesunate on the expression of pro-inflammatory cytokines in human sebaceous gland cells induced by Propionibacterium acnes

目的:研究青蒿琥酯(Art)对痤疮丙酸杆菌(P.acnes)诱导的人皮脂腺细胞(SZ95)促炎症因子表达的影响。方法:CCK8法检测不同浓度Art对人皮脂腺细胞活力的影响,筛选出适宜浓度的Art;RT-PCR、ELISA法检测肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β的表达。结果:Art在25~100μmol/L剂量范围内以剂量依赖关系抑制了痤疮丙酸杆菌诱导的人皮脂腺细胞分泌促炎症因子TNF-α、IL-1β mRNA和蛋白的表达(P<0.05)。结论:Art可抑制痤疮丙酸杆菌诱导的人皮脂腺细胞分泌促炎症因子TNF-α和IL-1β。

Objective:To investigate the effect of artesunate on the expression of pro-inflammatory cytokines in human sebaceous gland cells(SZ95)induced by Propionibacterium acnes(P.acnes).Methods:CCK8 method was performed to measure the activity of SZ95 human sebaceous gland cells affected by artesunate under different concentrations.The appropriate concentrations of artesunate were selected.RT-PCR and ELISA were then performed to measure the expression levels of TNF-αand IL-1β.Results:Artesunate inhibited the expression of TNF-αand IL-1βin SZ95 cells induced by P.acnes in a dosedependent manner(25~100μmol/L)at the mRNA and protein levels(P<0.05).Conclusion:Artesunate can inhibit the secretion of pro-inflammatory cytokines(TNF-αand IL-1β)in SZ95 cells induced by P.acnes.