基于无掩模光刻的生物透镜阵列组装方法

Assembly method of biological lens array based on maskless lithography

在微纳光学领域,透明的介质微球和活体生物细胞都因具备光学成像能力,而受到研究者越来越多的关注.然而,由于其尺寸和生物活性限制,很难将它们直接集成到光学系统上.基于光镊、声镊等多物理场方法虽然可以操作微球和细胞用于成像,但是对成像样本往往有特殊的要求.为此,本文提出了基于无掩模光刻的微球/细胞透镜阵列模块制备方法.利用自然沉降原理,分别将SiO_(2)微球和MCF-7细胞组装在水凝胶微孔阵列模块上形成微透镜阵列.实验和仿真结果表明,嵌入在水凝胶中的SiO_(2)微球阵列仍然保持其超分辨成像能力, MCF-7细胞可实现图像的放大.由于是阵列化成像,因此能够获得比单个微球或细胞更大的观测视野.本文所提出的模块化成像方法,有望应用于生物光子器件及在体光学成像领域.

In the field of micro and nano optics,transparent dielectric microspheres and living biological cells have attracted more and more attention due to their optical imaging capabilities.However,it is difficult to integrate them directly onto optical systems because of their size and bioactivity limitations.Although multi-physics field methods based on optical tweezers and acoustic tweezers can manipulate microspheres and cells for imaging,they tend to have specific requirements for imaging samples.Here,we proposed a method for the preparation of microsphere/cellular lens array modules based on maskless lithography.Natural sedimentation principle is utilized to form microlens arrays by assembling SiO_(2) microspheres and MCF-7 cells on the hydrogel microporous module.The experimental and simulation results show that the SiO_(2) microsphere array embedded in the hydrogel still maintains its superresolution imaging capability,and the MCF-7 cells can achieve the image magnification.As a result of the arrayed imaging,a larger viewing field can be obtained than that of individual microspheres or cells.The modular imaging method proposed in this paper is expected to be applied in the field of biophotonic devices and in vivo optical imaging.