沙棘发酵茶对高脂饮食大鼠血脂和肠道菌群的影响

Effects of sea buckthorn fermented tea on blood lipid and gut microbiota in high-fat diet rats

探究沙棘发酵茶对高脂血症大鼠血脂和肠道菌群的影响。将健康的SD雄性大鼠随机分为4组:对照组(NC,n=8)、模型组(HF,n=8)、沙棘组(HFS,n=8)和发酵组(HFF,n=8)。实验为期8周,最后1周收集粪便进行肠道菌群高通量测序。结束后,收集血清和肝脏进行测定。结果表明,沙棘发酵茶能显著降低高脂饮食大鼠血清中总胆固醇、甘油三酯和低密度脂蛋白的含量,升高血清中高密度脂蛋白的含量,缓解高脂饮食导致的肝脏损伤。测序结果显示,沙棘发酵茶能显著提高大鼠肠道菌群的丰度和均匀度,有益菌相对丰度升高,致病菌群得到抑制,有效缓解了高脂饮食引起的肠道菌群紊乱。综上,沙棘发酵茶能有效改善高脂饮食引起的大鼠血脂水平、肝损伤及肠道菌群紊乱。

This study aimed to explore the influence of sea buckthorn fermented tea on blood lipid and gut microflora in hyperlipidemia rats.Healthy male SD rats were randomly divided into four groups:normal control group(NC,n=8),hyperlipidemia model group(HF,n=8),sea buckthorn group(HFS,n=8)and fermented tea group(HFF,n=8).The experiment was conducted for eight weeks.Feces were collected for high-throughput sequencing of gut microbiota in the last week.After executed,the serum and liver were collected for blood lipid detection and pathological analysis.The results showed that sea buckthorn fermented tea could significantly reduce the levels of cholesterol,triglyceride and low density lipoprotein in hyperlipidemia rats,increase the levels of high density lipoprotein,and alleviate the liver damage caused by high fat diet.Sequencing analysis demonstrated that sea buckthorn fermented tea could significantly improve the abundance and uniformity of the intestinal flora in rat,increase the relative abundance of beneficial bacteria,inhibit the pathogenic bacteria,and restored gut microbiota disorder caused by high-fat diet.In conclusion,sea buckthorn fermented tea could be a potentially effective strategy to alleviate blood lipid level,liver damage and the gut microbiota disturbance in hyperlipidemia rats.