七氟烷对幼年猕猴前额叶皮质基因表达的影响

Gene expression in rhesus macaques prefrontal cortex after multiple sevoflurane exposures

目的:分析研究猕猴多次七氟烷麻醉后的差异表达基因。方法:猕猴在出生后第7天、第21天和第35天接受了3次七氟烷麻醉暴露。在第3次七氟烷麻醉后立即收集前额叶皮层进行RNA测序分析。结果:转录组的差异表达基因分析显示,上调基因主要富集在胚胎发育的正向调控、牙齿发育、胚胎前肢的形态发生中,而下调基因主要富集在对转录激活因子活性、RNA聚合酶Ⅱ转录调控区序列特异性结合和MAPK信号通路的调节中。结论:通过RNA测序技术分析研究猕猴多次七氟烷麻醉后前额叶皮层的差异表达基因,为之后研究七氟烷麻醉引起的神经毒性研究筛选出关键基因,对全身麻醉机制研究提供有力依据。

AIM:To analyze differentially expressed genes after multiple sevoflurane anesthesia in rhesus macaques was studied.METHODS:Rhesus macaques received three anesthetic exposures to sevoflurane on postnatal day(P)7 and then on P21 and P35.RNA sequencing analysis was used in the studies.RESULTS:Transcriptomic analysis of differentially expressed genes(DEGs)revealed that up-regulated genes were mainly enriched in the regulation of positive regulation of gastrulation and odontogenesis,while down-regulated genes were primarily enriched in the regulation of transcriptional activator activity, RNA polymeraseII transcription regulatory region sequencespecificbinding, and MAPK signaling pathway. CONCLUSION:Differentially expressed genes (DEGs)were analyzed in the prefrontal cortex of rhesusmacaques after multiple sevoflurane exposures byRNA sequencing to screen essential genes for subsequentstudies on neurotoxicity induced by sevoflurane,providing a solid basis for the study of themechanism of general anesthesia.