高通量技术分析‘砂蜜豆’甜樱桃不同组织内生细菌多样性

Diversity Analysis of Endophytic Bacterial Community in Different Tissues of‘Summit’Sweet Cherry Using High-throughput Sequencing

为探明甜樱桃内生细菌种群结构及多样性,以‘砂蜜豆’甜樱桃根、主干树皮和枝条3个组织的内生细菌为研究对象,对其16S rRNA V3~V4区进行高通量测序。结果表明,‘砂蜜豆’甜樱桃不同组织内生细菌群落多样性及功能存在较大差异。不同组织中共获得有效序列9665813条,聚类为1386个OTU(operational taxonomic units),隶属于20个门、43个纲、79个目、162个科和346个属。在门水平,变形菌门(Proteobacteria)在各组织中的丰度均最高,是最优势菌门。在属水平,根部内生细菌的优势菌属为链霉菌属(Streptomyces)、类固醇杆菌属(Steroidobacter)和乳酸杆菌属(Lactobacillus);枝条内生细菌中鞘氨醇单胞菌(Sphingomonas)的相对丰度较高;主干树皮内生细菌的优势菌属为拟杆菌属(Bacteroides)。PICRUSt(phylogenetic investigation of communities by reconstruction of unobserved states)基因预测表明,内生细菌功能主要涉及多糖、萜类和酮类、酶及维生素等次生代谢产物的生物合成。综上所述,甜樱桃内生细菌具有丰富的种群多样性,为今后开发利用甜樱桃内生细菌资源奠定了基础。

In order to investigate the microbial diversity of endophytic bacteria community in different tissues of sweet cherry,the V3~V4 regions of endophytic bacterial 16S rRNA from‘Summit’sweet cherry root,bark and branch were sequenced by Illumina high-throughput sequencing technology.The results showed that the diversity and function of endophytic bacterial community in different tissues of sweet cherry were significantly different.A total of 9665813 valid sequences and 1386 OTUs were obtained from different tissues of sweet cherry.Species classification showed that endophytic bacteria in sweet cherry belonged to 20 phyla,43 classes,79 orders,162 families and 346 genera.At the level of phylum,Proteobacteria was the dominant group,which was simultaneously found in different tissues.At genus level,the predominant genera in root were Streptomyces,Steroidobacter and Lactobacillus.The predominant genera in branch was Sphingomonas,and in bark was Bacteroides.PICRUSt(phylogenetic investigation of communities by reconstruction of unobserved states)function prediction analysis showed that the functions of endophytic bacteria in different tissues were involved in the biosynthesis of secondary metabolites such as polysaccharides,terpenes,ketones,enzymes and vitamins et al..The reults showed that endophytic bacteria in sweet cherry had rich population diversity,which laid a foundation for the development and utilization of endophytic bacteria resources in sweet cherry in the future.