基于碳纳米管/聚吡咯的细菌表面印迹传感器的构建与应用

Construction and application of carbon nanotube/polypyrrole-based bacterial surface imprinted sensor

构建了一种简单快速的“非空腔式”细菌表面印迹(SPBIP)阻抗传感器,用于对沙门氏菌进行超灵敏检测。SPBIP传感器由吡咯(功能单体)、单壁碳米管(SWNT,纳米调控剂)和沙门氏菌(模板)一步电聚合到玻碳电极上,去除细菌模板后,在聚合物基质表面形成“非空腔式”的印迹位点,使目标细菌可特异性的重新结合,由此产生的电极表面阻抗变化可用于检测目标细菌。研究了SWNT用量、聚合圈数、洗脱剂、洗脱时间和识别时间等对传感器识别能力的影响。在最佳条件下,该传感器可用于检测10~1×10^(7)CFU/mL沙门氏菌且检出限为3.5 CFU/mL。该传感器可用于饮用水和橙汁样品检测,回收率在95.4%~109.5%之间。

A simple and fast"non-hole"bacteria surface imprinted(SPBIP)impedance sensor was constructed for ultrasensitive detection of Salmonella.The SPBIP sensor was prepared by one step electropolymerization of pyrrole(functional monomer),single-walled carbon nanotube(SWNT,nano-modulator),and Salmonella(template)onto a glassy carbon electrode.After removing the bacterial template,"non-hole"imprinted sites were formed on the surface of the polymer matrix,allowing the target bacteria to be specifically recognized.The resulting changes in the electrode surface impedance could be used to detect the target bacteria.The effects of the amount of SWNT,polymerization cycles,eluents,elution time and recognition time on the recognition ability of the sensor were investigated.Under the optimal conditions,the sensor could be used to detect 10~1×107 CFU/mL Salmonella with the limit of detection of 3.5 CFU/mL.The sensor could be used for the detection of salmonella in drinking water and orange juice samples with the recoveries ranging from 95.4%to 109.5%.