高效液相色谱串联质谱法测定化妆品中全氟辛基磺酸

Determination of perfluorooctane sulfonic acid in cosmetics by high-perfor⁃mance liquid chromatography-tandem mass spectrometry

建立了高效液相色谱-串联质谱(HPLC-MS/MS)测定化妆品中全氟辛基磺酸(PFOS)的方法。甲醇超声提取,HPLC-MS/MS法定量PFOS。采用Agilent Poroshell 120 EC-C18(100 mm×2.1 mm,2.7μm)色谱柱、以0.1%甲酸水溶液(A)-甲醇(B)为流动相进行梯度洗脱,负离子多反应监测(MRM)模式检测。结果显示,在1~100μg/L范围内PFOS线性关系良好,相关系数r^(2)>0.9998;检出限与定量限分别为0.015和0.050 mg/kg;平均回收率为98.7%~112.5%,相对标准偏差(RSDs)均小于5%。该方法适用于化妆品中PFOS的测定。

A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was developed for the determination of perfluorooctane sulfonic acid(PFOS)in cosmetics.The cosmetic samples were extracted with methanol by ultrasonication,and PFOS was quantified by HPLC-MS/MS.The samples were detected in negative multiple reaction monitoring(MRM)mode using an Agilent Poroshell 120 EC-C18(100 mm×2.1 mm,2.7μm)column with a gradient elution using 0.1%formic acid aqueous solution(A)-methanol(B)as the mobile phase.The results showed that the linearity of PFOS was good in the range of 1-100μg/L with the correlation coefficient r^(2)larger than 0.9998;the limits of detection and limits of quantification were 0.015 and 0.050 mg/kg,respectively.The average recoveries were 98.7%-112.5%,and the relative standard deviations(RSDs)were less than 5%.The method is suitable for the determination of PFOS in cosmetics.