响应曲面法优化金水六君煎回流提取工艺

Optimization of Reflux Extraction Process of Jinshui Liujun Decoction by Response Surface Methodology

目的:优选金水六君煎中阿魏酸和橙皮苷回流提取工艺。方法:在单因素试验基础上,以提取时间、液料比、提取次数为影响因素,以阿魏酸和橙皮苷提取率为考察指标,采用响应曲面法优化金水六君煎回流提取工艺。结果:金水六君煎的最佳回流提取工艺为提取1.5 h、液料比12∶1,提取2次。在此工艺条件下,重复3次试验,阿魏酸提取率为60.59%,RSD为0.34%;橙皮苷提取率为59.67%,RSD为2.18%。结论:响应曲面法优化得到的金水六君煎最佳回流提取工艺参数稳定可靠,具有可行性和实用性。

Objective:To optimize the reflux extraction process of ferulic acid and hesperidin from Jinshui Liujun Decoction.Methods:On the basis of single factor experiment,the response surface methodology was used to optimize the reflux extraction process of Jinshui Liujun Decoction with reflux time,liquid-to-material ratio,and reflux times as influencing factors,and extraction rate of ferulic acid and hesperidin as the investigation indicator.Results:The optimal conditions of the reflux extraction process of Jinshui Liujun Decoction were as follows:reflux time of 1.5 hours,the liquidto-material ratio of 12:1,and twice reflux.Experiments were repeated three times under the process conditions,and the extraction rates of ferulic acid and hesperidin were 60.59%and 59.67%,with RSDs of 0.34%and 2.18%,respectively.Conclusion:The optimized reflux extraction process of Jinshui Liujun Decoction by response surface methodology is accurate and reliable,and it is feasible and practical.