血管紧张素转换酶2(ACE2)基因在家猫不同组织中的表达差异分析

Expression Difference Analysis of Angiotensin Converting Enzyme 2(ACE2)Gene in Different Tissues of Domestic Cats

【目的】分析血管紧张素转换酶2(ACE2)基因在家猫不同组织中的相对表达量及特定组织中的蛋白定位,为后续以ACE2基因为受体的疫病防控提供理论基础。【方法】采用qRT-PCR技术检测ACE2基因在家猫8个组织(心脏、肝脏、脾脏、肺脏、肾脏、胰腺、小肠和肠系膜淋巴结)中的mRNA相对表达水平,并利用免疫组织化学法检测家猫肾脏、胰腺和小肠中ACE2蛋白的组织定位。【结果】ACE2基因在家猫肾脏、胰腺和小肠组织的表达量极显著高于肺脏组织(P<0.01),在心脏、脾脏和肠系膜淋巴结组织的表达量极显著低于肺脏组织(P<0.01);不同性别家猫的ACE2表达量存在一定差异,但不显著;ACE2蛋白在小肠组织中的表达量最高,其次是肾脏,在胰腺的表达量最低。【结论】家猫ACE2基因在各组织中广泛分布且存在一定的表达差异。

[Purpose]To analyze the relative expression level of angiotensin converting enzyme 2(ACE2)gene in different tissues of domestic cats and the protein localization in specific tissues,providing the theoretical basis for the prevention and control of diseases with ACE2 gene as the receptor.[Methods]qRT-PCR technology was used to test the relative mRNA expression level of ACE2 gene in eight domestic cat tissues(heart,liver,spleen,lung,kidney,pancreas,small intestine and mesenteric lymph nodes),and the tissue localization of ACE2 protein in the kidney,pancreas and small intestine was tested by immunohistochemistry.[Results]The expressions of ACE2 gene in kidney,pancreas and small intestine tissues were extremely significantly higher than in lung tissue(P<0.01),while the expressions in heart,spleen and mesenteric lymph nodes were extremely significantly lower than in lung tissue(P<0.01).There were differences of ACE2 expression between different sexes,but the differences were not significant;ACE2 protein was the highest in small intestine,followed by kidney and the lowest in pancreas.[Conclusion]The ACE2 gene of domestic cat is widely distributed in various tissues and there are some differences in expression.