夜光藻高效培养方法建立及其有性繁殖发生机制的初步研究

Establishment of a high-efficiency culture method for Noctiluca scintillans and a preliminary study of its sexual reproduction mechanism

夜光藻(Noctiluca scintillans)是典型的赤潮生物,在世界多个海域形成过大面积赤潮,对海洋生态系统构成一定的威胁。夜光藻赤潮的发生不仅受外界环境因子的影响,其有性繁殖的方式在赤潮发生过程中也起着重要作用。本研究从连云港海域分离获得一株夜光藻,利用自行设计的饵料藻投喂装置,以天然海水为培养基,在温度20~25℃范围内实现了夜光藻的室内长期培养,并观察记录了夜光藻配子发生全过程。研究结果表明,夜光藻不同生长阶段培养体系中配子母细胞与营养细胞密度变化趋势基本一致,显示夜光藻的有性繁殖发生与种群丰度存在一定的相关性,但它们之间的因果关系还有待进一步探讨。本研究结果加深了对夜光藻有性繁殖的认识,有助于阐明其种群增长模式,并深入理解其赤潮暴发机制。

Noctiluca scintillans is a typical red tide organism and frequently forms severe red tides in coastal waters around the world,posing severe threats to marine ecosystems.Many studies have demonstrated that the occurrence of red tide due to N.scintillans is affected by external environmental factors,but its sexual reproduction also plays an important role.In this study,a strain of N.scintillans was isolated from the coastal waters of Lianyungang,Jiangsu Province,China,and a cultivation and feeding device was designed.An efficient laboratory cultivation method for N.scintillans was achieved across the temperature range of 20~25℃,and the entire gametogenesis process was observed.Furthermore,we found that the trends of N.scintillans gametogenic cell growth are consistent with those of trophonts at different growth stages.These results indicate that there is a correlation between the occurrence of sexual reproduction and the population abundance of N.scintillans;however,the causal relationship between them remains to be further explored.Generally,this study further improves our understanding of the sexual reproduction of N.scintillans,clarifies its population growth pattern,and provides insights into the mechanism of its role in red tide formation.