鲍姆桑黄子实体提取物的体外细胞毒活性及其化学成分分析

Characterization of cytotoxicity and chemical constituents of extracts of Sanghuangporus baumii basidiomata

本研究比较分析了鲍姆桑黄子实体乙酸乙酯萃取物和乙醇分离物的体外细胞毒活性及其化学成分差异,探索其抗肿瘤特征成分。采用CCK-8法测定了鲍姆桑黄子实体乙酸乙酯萃取物和乙醇分离物对5种人癌细胞增殖的抑制活性,结果发现,乙酸乙酯萃取物对5种人癌细胞增殖均表现出良好的细胞毒活性,而乙醇分离物对NCI-H1299细胞表现出一定的活性。通过超高效液相色谱-串联四极杆飞行时间高分辨率质谱(UPLC-Q-TOF-MS)对萃取物的主要成分进行定性分析,共鉴定出38种成分,包括26种多酚和12种脂肪酸,其中多酚主要为吡喃酮hispidin及其衍生物,乙酸乙酯萃取物中丰度较高的多酚成分包括hispidin、hispolon、davallialactone、osmundacetone、phelligridins C和D以及pinillidine。

The inhibitory effects of EtOAc fraction and ethanolic extracts of Sanghuangporus baumii basidiomata on the proliferation of tumour cells were detected by CCK-8 method.The results showed that the EtOAc fraction exhibits good inhibitory activities against NCI-H1299,SGC-7901,HepG2,MDA-MB-231 and HeLa cells after 48 h treatment,while the ethanolic extract shows good inhibitory activity against NCI-H1299 cell.The major chemical constituents of the extracts were analyzed by using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)in the negative ion mode.In total,26 phenolic compounds and 12 fatty acids were identified,and the main phenolic compounds were hispidin and its derivatives.The phenolic components with higher content in EtOAc fraction were mainly composed of hispidin,hispolon,davallialactone,osmundacetone,phelligridins C and D,and pinillidine.