MS-222对大黄鱼(Larimichthys crocea)麻醉保活运输效果研究

EFFECT OF ANESTHETIC TRANSPORTATION OF LARIMICHTHYS CROCEA IN MS-222

为优化大黄鱼(Larimichthyscrocea)麻醉运输技术,提高保活率,以不同浓度MS-222麻醉剂对其运输存活率、呼吸代谢、鱼体生理生化指标的影响展开研究。结果显示:(1)12 h模拟运输后,10mg/L麻醉组大黄鱼存活率显著高于其他实验组(P<0.05),30mg/L麻醉组积累死亡率已高于50%;(2)呼吸频率随麻醉剂浓度的升高显著降低(P<0.05),各组水体溶解氧含量随运输时长增加均呈下降趋势;(3)10和20 mg/L麻醉组大黄鱼复苏率分别为89%±10%和63%±19%,且均显著高于30 mg/L麻醉组(P<0.05);(4)随麻醉剂浓度的升高,大黄鱼血清中皮质醇、谷草转氨酶、乳酸脱氢酶、葡萄糖-6-磷酸酶水平逐渐降低,其肌肉乳酸水平显著降低(P<0.05),其肌糖原水平显著升高(P<0.05)。结果表明:高浓度(30mg/L)MS-222处理会引起大黄鱼呼吸抑制,造成运输存活率下降,不适合应用于大黄鱼的保活运输;低浓度(10mg/L)MS-222处理可以达到降低鱼体拥挤胁迫、应激反应及呼吸代谢的效果,并可以有效降低大黄鱼死亡率,保活时间可达12 h。

To optimize the anesthesia transportation technology and improve the survival rate of Larimichthys crocea,the effects of different concentrations of MS-222 anesthetics on the survival rate and respiratory metabolism,and physiological and biochemical indices of L.crocea were studied.Results show that after 12 h simulated transportation,the survival rate of 10 mg/L anesthesia group was the highest,which was significantly higher than that of other experimental groups(P<0.05),and the cumulative mortality of 30 mg/L anesthesia group was higher than 50%.With the increase of the MS-222 concentration,the respiratory rate decreased significantly(P<0.05),and the dissolved oxygen content in each group showed a decreasing trend when transportation time increased.The recovery rates of L.crocea in 10 and 20 mg/L anesthesia groups were 89%±10%and 63%±19%,respectively,and that in the 30 mg/L anesthesia group was significantly lower than that in other two anesthesia groups(P<0.05).With the increasing anesthetic concentration in different groups,the levels of cortisol,aspartate aminotransferase,lactate dehydrogenase,and glucose-6-phosphatase in serum of L.crocea gradually decreased after transportation.With the increase of the concentration MS-222,the muscle lactic acid content decreased significantly(P<0.05),but the muscle glycogen content increased significantly(P<0.05).Therefore,the high concentration of MS-222(30 mg/L)could inhibit the respiratory function of L.crocea,thus causing the decrease of the survival rate,which is not suitable for transportation of L.crocea.However,the low concentration of MS-222(10 mg/L)could reduce the crowding stress,lower the stress response,and promote the respiratory metabolism,and eventually,increase the survival rate of L.crocea and extend the survival time up to 12 hours.