基于RNA-Seq分析茶树新品系‘春绿’生物信息

RNA-Seq Analysis on A New Tea Strain,Chunlv

【目的】为加深对茶树新品系‘春绿’分子机制及优异性状的遗传基础的了解。【方法】以茶树品种(系)‘福云6号’和春绿叶片为材料,利用第二代测序技术,构建基因转录本。【结果】获得新品系春绿的Clean reads达到47.37 M,比对照福云6号少0.76 M;共拼装成41159条unigenes,其中31706条unigenes能被GO数据库成功注释,分别涉及到3651个GO组。差异显著基因有7042条,分别为23个生物学过程的GO组、20个细胞成分的GO组及21个基于分子功能的GO组。KEGG富集分析发现,有8657个基因注释到191个代谢通路中,这些基因中有1277个显著差异基因。66条DEGs参与苯丙素的生物合成(ko00940),3条DEGs参与谷胱甘肽代谢(ko00480),40条DEGs参与卵母细胞减数分裂(ko04114),45条DEGs参与RNA降解(ko03018),14条DEGs参与角质、亚伯碱和蜡的生物合成(ko00073)。【结论】转录组测序分析结果阐明了春绿新品系生长发育的部分分子信息,对利用其进一步开展分子辅助育种等具有较为重要的意义。

【Objective】Genes associated with the molecular mechanisms and biological characteristics of the new tea strain Chunlv were analyzed.【Method】The second generation sequencing technology was applied to determine the full-length transcriptomes of Camellia sinensis Chunlv as well as Fuyun No.6 for comparison.【Result】Leaves from the tea bushes were collected for the transcript construction.There were 47.37 M clean reads found in Chunlv,0.76 M less than that in the control Fuyun No.6.Of the 41159 UniGenes synthesized on the genes of Chunlv,31706 could be annotated by the GO database involving 3651 groups.Showing 7042 significantly distinct GO genes,the groups included the ones of 23 biological processes,20 cellular components,and 21 molecular function-based genes.The KEGG analysis annotated 8657 genes into 191 metabolic pathways with 1277 significantly differed from one another.On the DEGs,66 were involved in the phenylpropyl biosynthesis(ko00940),3 in glutathione metabolism(ko00480),40 in oocyte meiosis(ko04114),45 in RNA degradation(ko03018),and 14 in Cutin,suberine,and wax biosynthesis.【Conclusion】The transcriptome sequencing elucidated some genetic information on the Chunlv tea cultivar which would facilitate future studies involving and breeding programs utilizing the molecular markers.