摘要
目的:探讨精胺对琥珀酰氨基咪唑(SAICAr)水平的影响及用于改善腺苷酸琥珀酸裂解酶(ADSL)缺陷症的效用。方法:通过标准品建立了SAICAr检测的质谱技术方法。以5μM精胺处理A549和MDA-MB-231细胞24小时后,收集细胞,通过质谱检测细胞中SAICAr的含量。结论:精胺可有效地降低SAICAr水平,其有望成为ADSL缺陷症治疗领域具有开发前景的新药。
Objective:To investigate the effects of spermine on the level of s uccinylaminimidazole(SAICAr)and its effect on improving adenylate succinase(ADSL)defects.Methods:A mass spectrometry technique for SAICAr detection was established by using standard materials.A549 and MDA-MB-231 cells were treated with 5µM spermidine for 24 h,and the SAICAr content in the cells was detected by mass spectrometry.Conclusion:Spermine can effectively reduce the level of SAICAr,and it is expected to be a promising new drug in the treatment of ADSL defects.
作者
黄楠
彭勃
徐蕾
潘武广
赵德志
郭蕾
敖妍冉
朱威
潘秋辉
HUANG Nan;PENG Bo;XU Lei;PAN Wuguang;ZHAO Dezhi;GUO Lei;AO Yanran;ZHU Wei;PAN Qiuhui(Tenth People’s Hospital Affiliated to Tongji University,Shanghai 200072,China;Jinan University,Guangzhou 510632,China;Children’s Medical Center,Shanghai Jiao Tong Universi ty School of Medicine,Shanghai 200127,China;Guangzhou Junh e Biotechnology Co.,LTD.,Guangzhou 510663,China)
基金
广东省海洋经济发展(海洋六大产业)专项资金项目(2021)51号资助(GDNRC[2021]051)。
