美洲南瓜Dof基因家族鉴定与表达模式分析

Identification and expression pattern analysis of Dof gene family in Cucurbita pepo

DNA结合单锌指(DNA binding with one finger,Dof)是植物所特有的转录因子,主要参与调控植物的生长发育、抗逆和品质等生命活动过程。为了鉴定美洲南瓜Dof基因家族成员和探究其表达模式,从全基因组水平筛选和分析了美洲南瓜CpDof家族成员、基因结构、蛋白保守基序和亲缘关系等,同时,通过转录组和荧光定量技术比较了不同成员在美洲南瓜中的时空表达与果实发育表达特性。结果表明,在美洲南瓜中存在58个Dof基因并广泛分布于全基因组;功能域Motif 1存在于所有的Dof基因中;其启动子序列中含有光响应、激素响应和防御应激等顺式作用元件;Dof基因在美洲南瓜中具有组织表达特异性和时空表达特异性。基于转录组和荧光定量验证结果,表明CpDof7、CpDof30和CpDof42等基因与美洲南瓜356和296果实发育密切相关。以上结果为进一步克隆和解析南瓜属Dof基因功能提供了有力支撑。

DNA binding with one finger(Dof)is a transcription factor unique to plants,mainly involved in regulating plant life processes,such as growth and development,stress resistance,and quality.In order to identify the members of the Dof gene family and explore its expression pattern analysis in pumpkin(Cucurbita pepo),this study screened and analyzed the members,gene structure,protein conservative motifs and genetic relationships of CpDof family from the whole genome,and compared the spatiotemporal expression and fruit development expression characteristics of different members through transcriptome and fluorescence quantitative techniques.The results showed that there were 58 Dof genes and widely distributed all over the different chromosomes;The functional domain Motif 1 exists in all Dof genes;The promoter sequence contains cis acting elements such as light response,hormone response,and defense against stress;Dof gene has tissue expression specificity and spatiotemporal expression specificity in Cucurbita pepo.Based on the results of transcriptome and fluorescence quantitative verification,CpDof7,CpDof30 and CpDof42 genes are closely related to the fruit development of pumpkin 356 and 296.This provides strong support for further cloning and analysis of the Dof gene function in Cucurbita species.