摘要
目的:探讨二吲哚吡啶基吡咯烷(DIPRD)对肺癌A549细胞增殖的影响。方法:获取肺癌A549细胞,分别予以不同浓度(0、6.25、12.5、25、50、100μg/mL)的DIPRD孵育,孵育24 h后,分别采用细胞计数(CCK-8)检测细胞活性,克隆形成实验检测细胞增殖,免疫蛋白印迹实验(WB)检测蛋白激酶B(Akt)、雷帕霉素机械靶蛋白(mTOR)、细胞外调节蛋白激酶(Erk)蛋白磷酸化水平。结果:25μg/mL及以上浓度的二吲哚吡啶基吡咯烷孵育后可明显抑制A549细胞活力和细胞增殖。25μg/mL及以上浓度的二吲哚吡啶基吡咯烷孵育后可明显下调p-Akt、p-mTOR、p-Erk表达。结论:DIPRD可以有效抑制肺癌A549细胞增殖,此种作用分子机制可能与抑制Akt、mTOR及Erk蛋白磷酸化水平存在关系。
Objective:To investigate the effect of DIPRD on cell proliferation of A549 lung cancer cells.Method:Lung cancer A549 cells were obtained for cell culture,and incubated with DIPRD at different concentrations(0,6.25,12.5,25,50,100μg/mL).After incubation for 24 h,cell activity was detected by cell counting(CCK-8),and cell proliferation was detected by clonal formation assay.Immunoblotting(WB)was used to detect the phosphorylation levels of protein kinase B(Akt),mechanical target of rapamycin(mTOR)and extracellular regulatory protein kinase(Erk).Results:A549 cell viability and proliferation were significantly inhibited by incubation with diindoline pyridinyl pyrrolidine at 25μg/mL or above.The expression of p-Akt,p-mTOR and p-Erk was significantly down-regulated after incubation with diindoline pyridinyl pyrrolidine at 25μg/mL or above.Conclusion:DIPRD can effectively inhibit the proliferation of lung cancer A549 cells,and this molecular mechanism may be related to the inhibition of Akt,mTOR and Erk protein phosphorylation levels.
作者
赵金霞
颜亮
陈永权
ZHAO Jin-xia;YAN Liang;CHEN Yong-quan(The First Affiliated Hospital of Wannan Medical College(Yijishan Hospital),Wuhu 241000,Anhui;School of Basic Medical Sciences Wannan Medical College,Wuhu 241001,Anhui)
出处
《安徽医专学报》
2023年第2期78-80,共3页
Journal of Anhui Medical College
基金
皖南医学院中青年科研基金(编号:WK2021F15)。