腹泻型肠易激综合征患者的血清IL-10、CCL16水平及其临床意义

Serum IL-10 and CCL16 levels in patients with diarrhea predominant irritable bowel syndrome and their clinical significance

目的探究腹泻型肠易激综合征(IBS-D)患者的血清IL-10、CC趋化因子配体16(CCL16)水平及其临床意义。方法选择2019年1月至2022年12月在来安家宁医院就诊的65例IBS-D患者设为IBS-D组,另选择67名同期健康体检者设为对照组。收集受试者的一般资料;采用ELISA法检测血清IL-10、CCL16水平;采用细菌三级鉴定法鉴别受试者粪便菌群(双歧杆菌、乳杆菌、拟杆菌和肠杆菌),并计算每克粪便中的菌群数量。对IBS-D患者进行IBS-D症状严重程度的评估,并根据严重程度总评分将IBS-D患者分为轻中度组和重度组,采用Pearson或Spearman相关分析法探究IBS-D患者的血清IL-10、CCL16水平与粪便菌群、IBS-D症状严重程度评分的相关性。结果与对照组相比,IBS-D组的血清IL-10、CCL16水平均显著升高(P均<0.05),粪便中肠杆菌数量显著增多(P<0.05),双歧杆菌、乳杆菌数量均显著减少(P均<0.05)。IBS-D患者的IBS-D症状严重程度总评分为(262.00±41.25)分,与轻中度组相比,重度组的血清IL-10、CCL16水平均显著升高(P均<0.05),粪便中肠杆菌数量显著增多(P<0.05),双歧杆菌、乳杆菌数量均显著减少(P均<0.05)。IBS-D患者的血清IL-10水平与血清CCL16水平呈正相关(r=0.390,P<0.05);血清IL-10、CCL16水平与粪便中双歧杆菌、乳杆菌数量均呈负相关(P均<0.05),与肠杆菌数量、IBS-D症状各项评分及严重程度总评分均呈正相关(P<0.05)。结论IBS-D患者的血清IL-10、CCL16水平升高,且与粪便中双歧杆菌、乳杆菌、肠杆菌及患者的IBS-D症状严重程度均显著相关。检测血清IL-10、CCL16水平可能有助于诊断IBS-D及评估病情严重程度,进而指导临床治疗。

Objective This paper intends to explore the serum levels of IL-10 and CC chemokine ligand 16(CCL16)in patients with diarrhea predominant irritable bowel syndrome(IBS-D)and their clinical significance.Methods Sixty-five IBS-D patients who were treated at Lai’an Jianing Hospital from January 2019 to December 2022 were selected and assigned to the IBS-D group,and 67 healthy individuals who underwent physical examinations during the same period were selected and assigned to the control group.The general information of the subjects was collected and the ELISA method was used to detect serum IL-10 and CCL16 levels.The fecal flora(Bifidobacterium,Lactobacillus,Bacteroides,and Enterobacteriaceae)in the feces of the subjects were identified by the three-stage bacterial identification method,and the number of flora in per gram of feces was calculated.The severity of IBS-D symptoms in IBS-D patients was evaluated,and the IBS-D patients were divided into mild to moderate and severe groups based on the total severity score.Pearson or Spearman correlation analysis was used to explore the correlation between serum IL-10 and CCL16 levels,fecal flora,and IBS-D symptom severity score in IBS-D patients.Results Compared with the control group,the levels of serum IL-10 and CCL16 in the IBS-D group are significantly higher(P<0.05),the number of Enterobacteria in feces is significantly increased(P<0.05),and the number of Bifidobacteria and Lactobacilli are significantly reduced(P<0.05).The total score of IBS-D symptom severity of patients with IBS-D is(262.00±41.25)points.Compared with the mild and moderate group,the levels of serum IL-10 and CCL16 in the severe group are significantly higher(P<0.05),the number of Enterobacteria in feces is significantly increased(P<0.05),and the number of Bifidobacteria and Lactobacilli are significantly reduced(P<0.05).There is a positive correlation between serum IL-10 level and serum CCL16 level in IBS-D patients(r=0.390 and P<0.05).Serum IL-10 and CCL16 levels are negatively correlated with the number of Bifidobacteria and Lactobacilli in feces(P<0.05),and positively correlated with the number of Enterobacteriaceae,IBS-D symptom scores,and the total severity score(P<0.05).Conclusions The serum levels of IL-10 and CCL16 in IBS-D patients are elevated,and are significantly related to Bifidobacteria,Lactobacilli,and Enterobacteriaceae in the feces and the severity of IBS-D symptoms in patients.Detecting serum IL-10 and CCL16 levels may help diagnose IBS-D and assess the severity of the condition,thereby guiding clinical treatment.