脱支酶解-压热法制备淮山薯RS3抗性淀粉的工艺优化及体外消化模拟评价

Process optimization and in vitro digestion simulation evaluation of Dioscorea opposita Thunb.RS3 resistant starch prepared by debranching enzymolysis⁃pressure heat method

以淮山薯为原料,通过脱支酶解-压热法制备RS_(3)抗性淀粉。以抗性淀粉得率为指标,在单因素试验的基础上通过正交试验优化制备工艺,并通过体外消化模拟试验评价RS_(3)抗性淀粉的消化性能。结果表明:最佳制备工艺为淀粉乳质量分数25%、普鲁兰酶添加量200 U/g(以干基淀粉质量计)、酶解时间12 h、老化时间18 h,在此条件下RS_(3)抗性淀粉得率为16.95%±0.22%;淀粉还原糖释放量为11.35%±0.20%,RS_(3)抗性淀粉还原糖释放量为8.42%±0.14%(P<0.05),表明RS_(3)抗性淀粉比淀粉抗消化能力更强。

Using Dioscorea opposita Thunb.as raw material,RS_(3) resistant starch was prepared by de⁃branching enzymolysis⁃pressure heat method.Based on the yield of RS_(3) resistant starch as an indicator,the preparation process was optimized through orthogonal experiments on the basis of single factor experi⁃ments,and the digestion performance of RS_(3) resistant starch was evaluated through in vitro digestion sim⁃ulation experiments.The results showed that the best preparation technology was as follows:starch milk mass fraction 25%,pullulanase addition 200 U/g(based on dry starch mass),enzymolysis time 12 h,aging time 18 h.Under these conditions,the yield of RS_(3) resistant starch was 16.95%±0.22%.The release of starch reducing sugar was 11.35%±0.20%,and the release of RS_(3) resistant starch reducing sugar was 8.42%±0.14%(P<0.05),indicating that RS_(3) resistant starch has stronger digestive resistance than starch.