吸腐薄口螨休眠体的形态和分子特征鉴定

Identification of the morphological and molecular characteristics of the Histiostoma sapromyzarum hypopus

目的利用形态学和分子学方法对吸腐薄口螨的休眠体进行鉴定,了解其形态和分子特征。方法采用光镜和扫描电镜方法对生姜样品中的吸腐薄口螨进行表面和超微结构观察,同时提取休眠体基因组DNA,测序分析cox1和ITS基因。结果吸腐薄口螨休眠体足4对,腹面凹而背面凸,体壁较硬,清晰可见前足体和后足体被背沟分开。足上有刚毛,背毛短,躯体末端清晰可见吸盘板,共4对。经PCR扩增得到基因片段,大小为417 bp,ITS序列长度为1613 bp。结论利用吸腐薄口螨休眠体光镜和电子显微镜观察结合cox1和ITS序列,可鉴定吸腐薄口螨的休眠体,为吸腐薄口螨的生物学研究奠定了基础。

Objective Morphological and molecular methods were used to identify Histiostoma sapromyzarum hypopus and to understand its morphological and molecular characteristics.Methods The Histiostoma sapromyzarum hypopus was isolated from the colected ginger samples,and its external morphology and ultrastructure were observed under light microscope and scanning electron microscope for identification.Meanwhile,genomic hypopus DNA was extracted for sequencing and analysis of cox1 and ITS genes.Results The Histiostoma feroniarum hypopus was composed of 4 pairs of feet,the ventral surface was concave but dorsally convex,the body wall was hard,and it was clearly seen that the forefoot and hindfoot bodies were separated by the dorsal sulcus.There were bristles on the feet,short dorsal hair,and a suction disc plate was clearly visible at the end of the soma in 4 pairs.The size of cox1 gene fragment obtained by PCR amplification was 417 bp,and the total sequence length of ITS was 1613 bp.Conclusion The morphology of the Histiostoma sapromyzarum hypopus under light microscope and electron microscope combined with the cox1 and ITS gene sequences,identify Histiostoma sapromyzarum hypopus,which provide a basis for biological studies of it.