血清PTX3、sTREM-1、SOCS3联合对细菌性脓毒症患者的诊断价值

Diagnostic value of serum PTX3,sTREM-1 and SOCS3 in patients with bacterial sepsis

目的探讨穿透素3(PTX3)、可溶性髓系细胞受体-1(sTREM-1)、细胞因子信号传导抑制因子3(SOCS3)在细菌性脓毒症患者血清中的水平变化及诊断价值。方法本研究选取2019年1月至2022年12月在本院治疗的细菌性脓毒症患者94例作为观察组,同期选取非感染性全身性炎症性反应综合征(SIRS)患者80例及健康体检志愿者100例分别纳入对照组和健康组。检测血清PTX3、sTREM-1、SOCS3水平;受试者工作特征曲线(ROC)评价血清PTX3、sTREM-1、SOCS3水平对细菌性脓毒症患者的诊断价值;采用多因素Logistic回归分析法分析细菌性脓毒症的影响因素。结果观察组患者血清PTX3、sTREM-1、SOCS3表达水平分别为(104.39±23.93)ng/mL、(136.17±41.46)ng/mL、(346.59±31.12)μg/L,高于对照组患者血清PTX3、sTREM-1、SOCS3表达水平(53.93±12.19)ng/mL、(69.17±14.46)ng/mL、(271.19±26.02)μg/L及健康组患者血清PTX3、sTREM-1、SOCS3表达水平(1.17±0.33)ng/mL、(10.15±3.17)ng/mL、(163.32±17.33)μg/L,三组间比较差异均有统计学意义(F=5.928,588.015,11.785,P<0.05);ROC结果显示,血清PTX3、sTREM-1、SOCS3水平预测细菌性脓毒症患者的曲线下面积(AUC)分别为0.867、0.878、0.893,对应的敏感度分别为90.43%、78.72%、81.91%,特异度分别为86.25%、88.75%、90.00%,三者联合预测细菌性脓毒症患者的AUC为0.957,敏感度为92.55%,特异度为85.00%;Logistic回归分析显示PTX3、sTREM-1、SOCS3水平升高均是细菌性脓毒症的危险因素。结论PTX3、SOCS3、sTREM-1在细菌性脓毒症患者血清中均呈明显高表达,三者联合检测有助于诊断疾病的发生。

Objective To investigate the changes in serum levels of pentraxin 3(PTX3),soluble triggering receptor expressed on myeloid cell 1(sTREM-1)and suppressor of cytokine signaling 3(SOCS3)in patients with bacterial sepsis and their diagnostic value.Methods This study selected 94 patients with bacterial sepsis who were treated in our hospital from January 2019 to December 2022 as the observation group,meantime,80 patients with non-infectious systemic inflammatory response syndrome(SIRS)and 100 healthy volunteers were selected to be included in the control group and the health group respectively.The serum levels of PTX3,sTREM-1 and SOCS3 were detected;the diagnostic value of serum PTX3,sTREM-1 and SOCS3 levels in patients with bacterial sepsis was evaluated by the working characteristic curve(ROC)of subjects;multivariate Logistic regression analysis was applied to analyze the influencing f0actors of bacterial sepsis.Results The expression levels of serum PTX3,sTREM-1 and SOCS3 in observation group were 104.39±23.93 ng/mL,136.17±41.46 ng/mL and 346.59±31.12μg/L,respectively.The expression levels of PTX3,sTREM-1 and SOCS3 were higher than those of control group:53.93±12.19 ng/mL,69.17±14.46 ng/mL and 271.19±26.02μg/L and the expression levels of serum PTX3,sTREM-1 and SOCS3 in healthy group were 1.17±0.33 ng/mL,10.15±3.17 ng/mL,163.32±17.33μg/L,and there were statistically significant differences among the three groups(F=5.928,588.015,11.785,P0.05);ROC results showed that the area under curve(AUC)of serum PTX3,sTREM-1 and SOCS3 levels in patients with bacterial sepsis was 0.867,0.878 and 0.893,respectively,with corresponding sensitivity of 90.43%,78.72%and 81.91%,and specificity of 86.25%,88.75%and 90.00%,respectively,the AUC of the combination for predicting bacterial sepsis was 0.957,the sensitivity was 92.55%,and the specificity was 85.00%;Logistic regression analysis showed that elevated levels of PTX3,sTREM-1 and SOCS3 were risk factors for bacterial sepsis.Conclusion PTX3,SOCS3 and sTREM-1 are highly expressed in the serum of patients with bacterial sepsis,and the combined detection of the three is helpful to diagnose the occurrence of the disease.