乙醛脱氢酶2在子宫内膜癌组织中的表达及其与患者临床特征的相关性

Expression of acetaldehyde dehydrogenase 2 in endometrial carcinoma and its correlation with clinical characteristics of patients

目的通过检测乙醛脱氢酶(ALDH)2、微小RNA(miR)-135b-3p在子宫内膜癌组织中表达情况,探究其与临床特征的相关性。方法选取西北妇女儿童医院2019年2月至2022年2月间手术切除的94例子宫内膜癌组织标本(EC组)和60例正常子宫内膜标本(对照组)。实时荧光定量PCR(qRT-PCR)技术检测两组子宫内膜组织中ALDH2 mRNA、miR-135b-3p表达变化,免疫组化法检测ALDH2蛋白阳性表达率。分析ALDH2 mRNA、miR-135b-3p表达水平与临床病理特征关系。结果EC组ALDH2 mRNA表达(0.67±0.15)低于对照组(1.05±0.12),miR-135b-3p表达水平(1.52±0.26)高于对照组(1.01±0.06)。EC组癌组织ALDH2蛋白阳性表达率为30.85%(29/94),低于对照组正常子宫内膜组织中ALDH2蛋白阳性表达率51.67%(31/60)(P<0.05)。子宫内膜癌患者ALDH2 mRNA、miR-135b-3p表达水平与FIGO分期、淋巴结转移、分化程度、子宫肌层浸润有关(均P<0.05),而与年龄、病理类型、绝经、HPV感染、初潮年龄、产次、肿瘤长径、BMI无关(均P>0.05)。结论子宫内膜癌组织中ALDH2表达下调、miR-135b-3p表达上调,二者可能参与肿瘤的发生、发展。

Objective To detect the expression of acetaldehyde dehydrogenase(ALDH)2 and microRNA(miR)-135b-3p in endometrial cancer(EC)tissues,and to explore their correlation with clinical characteristics.Methods 94 endometrial cancer tissue specimens(EC group)and 60 normal endometrial specimens(control group)were selected from Northwest Women's and Children's Hospital from February 2019 to February 2022.Real time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression changes of ALDH2 mRNA and miR-135b-3p in endometrial tissue of two groups,and immunohistochemistry was used to detect the positive expression rate of ALDH2 protein.The relationship between the expression levels of ALDH2 and miR-135b-3p and the clinicopathological characteristics was analyzed.Results The expression of ALDH2 mRNA in the EC group(0.67±0.15)was lower than that in the control group(1.05±0.12),and the expression level of miR-135b-3p(1.52±0.26)was higher than that in the control group(1.01±0.06).The positive expression rate of ALDH2 in cancer tissue of EC group was 30.85%(29/94),which was lower than 51.67%(31/60)in normal endometrial tissue of the control group(P<0.05).The expression levels of ALDH2 mRNA and miR-135b-3p in EC patients were related to International Federation of Obstetrics and Gynecology(FIGO)stage,lymph node metastasis,differentiation and myometrium invasion(all P<0.05),but not to age,pathological type,menopause,HPV infection,menarche age,parity,tumor length and BMI(all P>0.05).Conclusions In EC tissues,the expression of ALDH2 is down-regulated and the expression of miR-135b-3p is up-regulated,which may be involved in the occurrence and development of EC.